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Crystallographic and biochemical studies on dissimilatory sulfite reductases
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Resumo(s)
Life on earth is only possible through tightly interwoven material
transformations through various cycles. Carbon, nitrogen, fosforous
and sulfur, with a special interest in the latter, are essential
components of all living organisms and represent the most
important elements circulating within the biosphere.
During this circulation, sulfur can be found in various oxidation
states with transformations occurring both biological and
chemically. Dissimilatory sulfate reduction is one of those reactions,
where sulfate is reduced to the final product sulfide in order to
obtain energy for their metabolism. Sulfate reduction however, is
not a favourable energetic reaction, and so sulfate is initially
activated to adenosine-5’-phosphosulfate (APS) by ATP sulfurylase.
APS is then reduced to sulfite by APS reductase allowing the sulfite
reductase to reduce sulfite to the final product sulfide in a six
electron transfer reaction. This last step can occur in an assimilatory
or dissimilatory way.(...)
Descrição
Thesis dissertation presented to obtain a PhD degree in
Biochemistry at Instituto de Tecnologia Química e Biológica,
Universidade Nova de Lisboa
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Editora
Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica