Utilize este identificador para referenciar este registo: http://hdl.handle.net/10362/5453
Título: Role of a novel disulfide bridge within the all-beta fold of soluble Rieske proteins
Autor: Botelho, Hugo M.
Leal, Sónia S
Veith, Andreas
Prosinecki, Vesna
Bauer, Christian
Fröhlich, Renate
Kletzin, Arnulf
Gomes, Cláudio M.
Palavras-chave: Rieske ferredoxin
Iron–sulfur cluster
Protein stability
Data: 29-Out-2009
Editora: Springer
Citação: Botelho, H. M., Leal, S. S., Veith, A., Prosinecki, V., Bauer, C., Frohlich, R., Kletzin, A., and Gomes, C. M. (2010) Role of a novel disulfide bridge within the all-beta fold of soluble Rieske proteins, J Biol Inorg Chem 15, 271-281
Resumo: Rieske proteins and Rieske ferredoxins are present in the three domains of life and are involved in a variety of cellular processes. Despite their functional diversity, these small Fe-S proteins contain a highly conserved all-beta fold, which harbors a [2Fe-2S] Rieske center. We have identified a novel subtype of Rieske ferredoxins present in hyperthermophilic archaea, in which a two-cysteine conserved SKTPCX(2-3)C motif is found at the C-terminus. We establish that in the Acidianus ambivalens representative, Rieske ferredoxin 2 (RFd2), these cysteines form a novel disulfide bond within the Rieske fold, which can be selectively broken under mild reducing conditions insufficient to reduce the [2Fe-2S] cluster or affect the secondary structure of the protein, as shown by visible circular dichroism, absorption, and attenuated total reflection Fourier transform IR spectroscopies. RFd2 presents all the EPR, visible absorption, and visible circular dichroism spectroscopic features of the [2Fe-2S] Rieske center. The cluster has a redox potential of +48 mV (25ºC and pH 7) and a pKa of 10.1 +/- 0.2. These shift to +77 mV and 8.9 +/- 0.3, respectively, upon reduction of the disulfide. RFd2 has a melting temperature near the boiling point of water (Tm = 99ºC, pH 7.0), but it becomes destabilized upon disulfide reduction (DeltaTm = -9ºC, DeltaCm = -0.7 M guanidinium hydrochloride). This example illustrates how the incorporation of an additional structural element such as a disulfide bond in a highly conserved fold such as that of the Rieske domain may fine-tune the protein for a particular function or for increased stability.
Descrição: Journal of Biological Inorganic Chemistry (2010)15: 271-281
URI: http://hdl.handle.net/10362/5453
Aparece nas colecções:ITQB: PBFS - Artigos em revista internacional com arbitragem científica

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
2010 - Botelho - JBIC.pdfMain article818,38 kBAdobe PDFVer/Abrir
2010 - Botelho - JBIC - supplementary.pdfSupplementary information1,95 MBAdobe PDFVer/Abrir

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.