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http://hdl.handle.net/10362/40318| Title: | Inhibition of cellular methyltransferases promotes endothelial cell activation by suppressing glutathione peroxidase 1 protein expression |
| Author: | Barroso, Madalena Florindo, Cristina Kalwa, Hermann Silva, Zélia Turanov, Anton A. Carlson, Bradley A. De Almeida, Isabel Tavares Blom, Henk J. Gladyshev, Vadim N. Hatfield, Dolph L. Michel, Thomas Castro, Rita Loscalzo, Joseph Handy, Diane E. |
| Keywords: | Biochemistry Molecular Biology Cell Biology |
| Issue Date: | 30-May-2014 |
| Abstract: | Background: Methylation of tRNASec facilitates the incorporation of selenocysteine at a UGA codon during translation. Results: Accumulation of the homocysteine precursor S-adenosylhomocysteine decreases tRNASec methylation, reducing glutathione peroxidase 1 expression and increasing oxidative stress-induced inflammatory activation of endothelial cells. Conclusion: Methylation modulates the expression of selenoproteins to regulate redox-dependent inflammatory pathways. Significance: Hypomethylation stress promotes a proatherogenic endothelial cell phenotype. |
| Description: | This work was supported, in whole or in part, by National Institutes of Health Grants HL067195, HL070819, HL048743, HL107192, and HL108630 (to J. L.); HL46457 and HL48743 (to T. M.); and GM061603 (to V. N. G.). This work was also supported by an American Heart Association postdoctoral fellowship grant (to H. K.) and by Portuguese Fundacao para a Ciencia e a Tecnologia Grants PTDC/SAU-ORG/112683/2009 (to R. C.) and SFRH/BD/73021/2010 (M. B.). |
| Peer review: | yes |
| URI: | http://www.scopus.com/inward/record.url?scp=84901724596&partnerID=8YFLogxK |
| DOI: | https://doi.org/10.1074/jbc.M114.549782 |
| ISSN: | 0021-9258 |
| Appears in Collections: | NMS: CEDOC - Artigos em revista internacional com arbitragem científica |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| J._Biol._Chem._2014_Barroso_15350_62.pdf | 1,3 MB | Adobe PDF | View/Open |
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