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Penicillium glabrum cork colonising isolates – preliminary analysis of their genomic similari
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Orientador(es)
Resumo(s)
Cork stopper manufacturing process includes an operation, known as
stabilisation, by which humid cork slabs are extensively colonised by fungi. The
effects of fungal growth on cork are yet to be completely understood and are
considered to be involved in the so called “cork taint” of bottled wine. It is
essential to identify environmental constraints which define the appearance of
the colonising fungal species and to trace their origin to the forest and/or as
residents in the manufacturing space. The present article correlates two sets of
data, from consecutive years and the same season, of systematic biologic
sampling of two manufacturing units, located in the North and South of Portugal.
Chrysonilia sitophila dominance was identified, followed by a high diversity of
Penicillium species. Penicillium glabrum, found in all samples, was the most
frequent isolated species. P. glabrum intra-species variability was investigated
using DNA fingerprinting techniques revealing highly discriminative polymorphic
markers in the genome. Cluster analysis of P. glabrum data was discussed in
relation to the geographical location of strains, and results suggest that P.
glabrum arise from predominantly the manufacturing space, although cork resident
fungi can also contrib
Descrição
Palavras-chave
Cork Penicillium glabrum DNA fingerprint fungal genomic similarity
Contexto Educativo
Citação
Editora
Revista Iberoamericana de Micologia