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Gene expression changes in the salivary glands of Anopheles coluzzii elicited by Plasmodium berghei infection
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Resumo(s)
Background: Malaria is a devastating infectious disease caused by Plasmodium parasites transmitted through the bites of infected Anopheles mosquitoes. Salivary glands are the only mosquito tissue invaded by Plasmodium sporozoites, being a key stage for the effective parasite transmission, making the study of Anopheles sialome highly relevant. Methods: RNA-sequencing was used to compare differential gene expression in salivary glands of uninfected and Plasmodium berghei-infected Anopheles coluzzii mosquitoes. RNA-seq results were validated by quantitative RT-PCR. The transmembrane glucose transporter gene AGAP007752 was selected for functional analysis by RNA interference. The effect of gene silencing on infection level was evaluated. The putative function and tertiary structure of the protein was assessed. Results: RNA-seq data showed that 2588 genes were differentially expressed in mosquitoes salivary glands in response to P. berghei infection, being 1578 upregulated and 1010 downregulated. Metabolism, Immunity, Replication/Transcription/Translation, Proteolysis and Transport were the mosquito gene functional classes more affected by parasite infection. Endopeptidase coding genes were the most abundant within the differentially expressed genes in infected salivary glands (P∈
Descrição
PMID: 26395987 WOS:000361802900001
Palavras-chave
Anopheles coluzzii Glucose transporter Plasmodium berghei RNA-seq RNAi Salivary glands Sporozoite Parasitology Infectious Diseases Biochemistry, Genetics and Molecular Biology (miscellaneous) SDG 3 - Good Health and Well-being