ITQB: SG - Artigos em revista internacional com arbitragem científica
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- The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stressPublication . Santos, Sandra P.; Yang, Yang; Rosa, Margarida T.G.; Rodrigues, Mafalda A.A.; De La Tour, Claire Bouthier; Sommer, Suzanne; Teixeira, Miguel; Carrondo, Maria A.; Cloetens, Peter; Abreu, Isabel A.; Romão, Célia V.; Instituto de Tecnologia Química e Biológica António Xavier (ITQB); Nature Publishing GroupThe bacterium Deinococcus radiodurans is highly resistant to several stress conditions, such as radiation. According to several reports, manganese plays a crucial role in stress protection, and a high Mn/Fe ratio is essential in this process. However, mobilization of manganese and iron, and the role of DNA-binding-proteins-under-starved-conditions during oxidative-stress remained open questions. We used synchrotron-based X-ray fluorescence imaging at nano-resolution to follow element-relocalization upon stress, and its dependency on the presence of Dps proteins, using dps knockout mutants. We show that manganese, calcium, and phosphorus are mobilized from rich-element regions that resemble electron-dense granules towards the cytosol and the cellular membrane, in a Dps-dependent way. Moreover, iron delocalizes from the septum region to the cytoplasm affecting cell division, specifically in the septum formation. These mechanisms are orchestrated by Dps1 and Dps2, which play a crucial role in metal homeostasis, and are associated with the D. radiodurans tolerance against reactive oxygen species.
- N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer CellsPublication . Zuhra, Karim; Tome, Catarina S.; Masi, Letizia; Giardina, Giorgio; Paulini, Giulia; Malagrino, Francesca; Forte, Elena; Vicente, Joao B.; Giuffre, Alessandro; Programme in Translational Medicine (iNOVA4Health); Instituto de Tecnologia Química e Biológica António Xavier (ITQB); MDPI - Multidisciplinary Digital Publishing Institute
- Hydrogen sulfide biochemistry and interplay with other gaseous mediators in mammalian physiologyPublication . Giuffrè, Alessandro; Vicente, João B.; Programme in Translational Medicine (iNOVA4Health); Instituto de Tecnologia Química e Biológica António Xavier (ITQB); HindawiHydrogen sulfide (H2S) has emerged as a relevant signaling molecule in physiology, taking its seat as a bona fide gasotransmitter akin to nitric oxide (NO) and carbon monoxide (CO). After being merely regarded as a toxic poisonous molecule, it is now recognized that mammalian cells are equipped with sophisticated enzymatic systems for H2S production and breakdown. The signaling role of H2S is mainly related to its ability to modify different protein targets, particularly by promoting persulfidation of protein cysteine residues and by interacting with metal centers, mostly hemes. H2S has been shown to regulate a myriad of cellular processes with multiple physiological consequences. As such, dysfunctional H2S metabolism is increasingly implicated in different pathologies, from cardiovascular and neurodegenerative diseases to cancer. As a highly diffusible reactive species, the intra- and extracellular levels of H2S have to be kept under tight control and, accordingly, regulation of H2S metabolism occurs at different levels. Interestingly, even though H2S, NO, and CO have similar modes of action and parallel regulatory targets or precisely because of that, there is increasing evidence of a crosstalk between the three gasotransmitters. Herein are reviewed the biochemistry, metabolism, and signaling function of hydrogen sulfide, as well as its interplay with the other gasotransmitters, NO and CO.