ITQB: BET - PhD Theses
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- Exploring Structural /functional relashionship in Type II NADH: quinone oxidoreductasesPublication . Sena, Filipa V.; Pereira, Manuela"Type II NADH:quinone oxidoreductases (NDH-2s) are membrane bound enzymes involved in respiratory chains. They contribute to the regeneration of NAD(P)+, allowing several metabolic pathways to proceed. These enzymes do not translocate charges across the membrane and thus do not contribute directly to the generation of the transmembrane difference in the electrochemical potential. NDH-2s are members of the so-called two- dinucleotide binding domain flavoprotein (tDBDF) superfamily, which are characterized by having NAD(P)H- and flavin-binding domains, with identical structural Rossmann folds. (...)"
- Exploring NADH:quinone oxidoreductasesPublication . Marreiros, Bruno Sousa Cardoso da Costa; Pereira, ManuelaThe work presented in this Thesis addressed the diversity of respiratory membrane enzymes with special focus on type I and type II NADH:quinone oxidoreductases. The searching for common denominators that characterize these enzymes in order to find minimal functional elements relevant for their operation was the main rational in this work.(...)
- Energy transduction by respiratory complex IPublication . Batista, Ana P.; Teixeira, Miguel; Pereira, Manuela M.The aim of the work presented in this dissertation was to provide a contribution to the understanding of the energy transducing mechanism of respiratory complex I. This enzyme is present in most bacteria and in all mitochondrial systems and it is characterized by its large number of subunits, its prosthetic groups (flavin and iron-sulfur centers), and its NADH:quinone oxidoreductase activity sensitive to specific inhibitors and coupled with charge translocation across the membrane.(...)
- The alternative complex III from Rhodothermus marinus - a prototype of a new family of quinol: electron acceptor oxidoreductasePublication . Refojo, Patrícia N.; Teixeira, Miguel; Pereira, Manuela M.The aim of the work presented in this thesis was the characterization of a complex with quinol: electron carrier oxidodoreductase activity present in the membranes of the thermohalophilic bacterium Rhodothermus (R.) marinus. The complexes involved in the R. marinus respiratory chain have been extensively studied in the past few years. Specifically, the purification and characterization of a complex I (NADH: quinone oxidoreductase), a complex II (succinate:quinone oxidoreductase) and of three different oxygen reductases from the heme-copper oxygen reductases superfamily have been performed. Since those oxygen reductases are unable to receive electrons from quinol molecules, the presence of a complex linking complexes I and II to the oxygen reductases was needed. In fact, a complex with quinol: HiPIP oxidoreductase activity was purified and partially characterized. The absence of the Rieske protein indicated that the complex isolated from R. marinus has a different composition when compared with the typical cytochrome bc1 complex.(...)