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Publicação
Office paper platform for bioelectrochromic detection of electrochemically active bacteria using tungsten trioxide nanoprobes
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Orientador(es)
Resumo(s)
Electrochemically active bacteria (EAB) have the capability to transfer electrons to cell exterior, a feature that is currently explored for important applications in bioremediation and biotechnology fields. However, the number of isolated and characterized EAB species is still very limited regarding their abundance in nature. Colorimetric detection has emerged recently as an attractive mean for fast identification and characterization of analytes based on the use of electrochromic materials. In this work, WO 3 nanoparticles were synthesized by microwave assisted hydrothermal synthesis and used to impregnate non-treated regular office paper substrates. This allowed the production of a paper-based colorimetric sensor able to detect EAB in a simple, rapid, reliable, inexpensive and eco-friendly method. The developed platform was then tested with Geobacter sulfurreducens, as a proof of concept. G. sulfurreducens cells were detected at latent phase with an RGB ratio of 1.10 ± 0.04, and a response time of two hours.
Descrição
This work was partially supported by the Portuguese Science Foundation (FCT-MEC) through the projects EXCL/CTM-NAN/0201/2012, Pest-C/CTM/LA0025/2013-14, Pest-C/EQB/LA0006/2013-14, PTDC/BBB-BEP/0753/2012 and the ERC Advanced Grant (INVISIBLE, contract number 228144). Geobacter sulfurreducens cells were kindly provided by Prof. Derek Lovley from University of Massachusetts (USA). We would like to thank to Dr. Daniela Nunes for the SEM images.
Palavras-chave
MICROBIAL FUEL-CELLS HYDROTHERMAL SYNTHESIS NANOWIRES GEOBACTER CONDUCTIVITY BIOFILMS OXIDE General