Fluorescent Probes Based on 7-(Diethylamino)quinolin-2(1H)-one Cucurbit[7]uril Complexes for Indicator Displacement Assays

Droguett, Kevin; Quintero, Guillermo E.; Basilio, Nuno; Fierro, Angélica; Pérez, Edwin G.; Aliaga, Margarita E.

http://hdl.handle.net/10362/187822

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Autores

Droguett, Kevin

Quintero, Guillermo E.

Resumo(s)

In this work, two 7-(diethylamino)quinolin-2(1H)-one derivatives (DQ1 and DQ2) were synthesized and characterized both structurally and physicochemically. The interaction of these derivatives with cucurbit[7]uril (CB7) was explored through combined experimental and in silico approaches, highlighting their unique behavior and practical potential. Experimentally, the complexes exhibited a 1:1 stoichiometry, as confirmed by fluorescence spectroscopy and isothermal titration calorimetry (ITC). Remarkably, the interaction with the CB7 macrocycle resulted in an unusual negative shift in the pKa values of the probes alongside a significant enhancement in fluorescence emission, quantum yield, and fluorescence lifetime. From a computational perspective, the molecular dynamics simulations (MD) demonstrated that hydrogen bonds play a critical role in maintaining the system’s stability. Finally, DQ2 is proposed as a probe for the indicator displacement assay (IDA) and was tested using methyl viologen (MV) as an analyte.

Descrição

Funding Information: This work was supported by Fondecyt grants N\u00B0 1210751 ( MEA ) and 1221030 ( AF ). KD acknowledges the ANID Doctoral Fellowship Folio N\u00B0 21210424. GEQ acknowledges the ANID Doctoral Fellowship Folio N\u00B0 21210698. Publisher Copyright: © 2025 The Authors. Published by American Chemical Society.