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Influence of inorganic carbon on purple phototrophic bacteria polyhydroxyalkanoates production under high reductive stress environment
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Resumo(s)
Fermented wastes rich in reduced compounds challenge anaerobic purple phototrophic bacteria (PPB) systems by causing redox imbalances. This study evaluated polyhydroxyalkanoates (PHA) production and CO2 fixation by PPB mixed cultures as means to balance internal redox, under four inorganic carbon (IC) availability conditions in a sequencing batch reactor. Culture selection under permanent IC presence promoted higher microbial diversity, but, strongly dependent on IC to balance internal redox, even when more oxidized substrates were supplemented. Increasing IC limitation favoured Rhodopseudomonas palustris, revealing its redox balancing capability independently of IC fixation. PHA contents of 20–29% gPHA/gVSS were achieved across all IC availabilities, however, when IC was present, growth was promoted over PHA production, indicating a preferable electron balance through CO2 fixation. Nevertheless, cultures selected under feast-famine IC exhibited a preference for PHA accumulation under IC limitation, suggesting IC tuning as a potential growth-controlling strategy to boost phototrophic PHA production using reduced feedstocks.
Descrição
Funding Information:
This work was supported by national funds from FCT - Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. J.R.A. also acknowledges the financial support of FCT - Fundação para a Ciência e a Tecnologia through the Ph.D. grant DFA/BD/8201/2020.
Publisher Copyright:
© 2025 The Author(s)
Palavras-chave
Anaerobic systems Butyric acid Carbohydrates IC fixation Polyhydroxyalkanoates (PHA) Purple phototrophic bacteria (PPB) mixed cultures Reductive stress Bioengineering Environmental Engineering Renewable Energy, Sustainability and the Environment Waste Management and Disposal SDG 7 - Affordable and Clean Energy