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Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes
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Resumo(s)
The aim of this study was to explore the viability of the dual production of poly(3-hydroxybutyrate) (PHB) and exopolysaccharides (EPS) by seven microbiomes rich in cyanobacteria. Our initial experiments involved to screen for EPS-producing candidates and examine the impact of salinity and acetate on EPS synthesis. Salinity's known influence on EPS production and acetate's role in enhancing PHB production guided our parameter selection. Surprisingly, neither the introduction of an external carbon source (acetate) nor exposure to an abiotic stressor (salt) significantly altered EPS synthesis rates, which ranged from 25 to 150 mg·L−1, or its composition, with glucose being the dominant sugar component. Scaling up to a 3 L photobioreactor, we achieved simultaneous biopolymer production, reaching up to 205 mg·L−1 EPS and 87 mg·L−1 PHB. Additionally, the presence of uronic acid in the EPS facilitated biomass flocculation, streamlining the separation process, and potentially reducing associated time and costs.
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Funding Information:
This research was supported by the European Union’s Horizon 2020 research and innovation programme under the grant agreement No 101000733 (project PROMICON). B. Altamira-Algarra thanks the Agency for Management of University and Research Grants (AGAUR) from the Government of Catalonia for her grant [FIAGAUR_2021]. E. Gonzalez-Flo would like to thank the European Union-NextGenerationEU, Ministry of Universities and Recovery, Transformation and Resilience Plan for her research grant [2021UPF-MS-12]. J. Garcia acknowledges the support provided by the ICREA Academia program. C. A.V. Torres and M. A.M. Reis acknowledges national funds from FCT - Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB.
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© 2025 The Authors
Palavras-chave
Biopolymer Bioprocess. Confocal Scanning Laser Microscope Photobioreactor Polyhydroxyalkanoate Synechoccocus sp. Synechocystis sp. Biotechnology Bioengineering Molecular Biology