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Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate
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Orientador(es)
Resumo(s)
Malaria remains an important and challenging infectious disease, and novel antimalarials are required. Benzyl isothiocyanate (BITC), the main breakdown product of benzyl glucosinolate, is present in all parts of Tropaeolum majus L. ( T. majus) and has antibacterial and antiparasitic activities. To our knowledge, there is no information on the effects of BITC against malaria. The present study evaluates the antimalarial activity of aqueous extracts of BITC and T. majus seeds, leaves, and stems. We used flow cytometry to calculate the growth inhibition (GI) percentage of the extracts and BITC against unsynchronized cultures of the chloroquine-susceptible Plasmodium falciparum 3D7 - GFP strain. Extracts and/or compounds with at least 70% GI were validated by IC50 estimation against P. falciparum 3D7 - GFP and Dd2 (chloroquine-resistant strain) unsynchronized cultures by flow cytometry, and the resistance index (RI) was determined. T. majus aqueous extracts showed some antimalarial activity that was higher in seeds than in leaves or stems. BITC's GI was comparable to chloroquine's. BITC's IC50 was similar in both strains; thus, a cross-resistance absence with aminoquinolines was found (RI < 1). BITC presented features that could open new avenues for malaria drug discovery.
Descrição
Funding Information: This research was funded by Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia through projects GHTMUID/04413/2020 and LA-REAL-LA/P/0117/2020. Publisher Copyright: © 2024 by the authors.
Palavras-chave
Plasmodium falciparum Tropaeolum majus L antimalarials benzyl isothiocyanate Infectious Diseases Parasitology Pharmacology, Toxicology and Pharmaceutics(all) Drug Discovery SDG 3 - Good Health and Well-being