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Synechocystis ferredoxin/ferredoxin-NADP+-reductase/NADP+ complex: Structural model obtained by NMR-restrained docking
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Orientador(es)
Resumo(s)
Abstract Ferredoxin (Fd) and ferredoxin-NADP+-reductase(FNR) are two terminal physiological partners of the photosynthetic
electron transport chain. Based on a nuclear magnetic resonance(NMR)-restrained-docking approach, two alternative structural models of the Fd–FNR complex in the presence of
NADP+ are proposed. The protein docking simulations were performed with the software BiGGER. NMR titration revealed a 1:1 stoichiometry for the complex and allowed the mapping of the interacting residues at the surface of Fd. The NMR chemical
shifts were encoded into distance constraints and used with theoretically
calculated electronic coupling between the redox cofactors to propose experimentally validated docked complexes.
Descrição
FEBS Letters 579 (2005) 4585–4590
Palavras-chave
Ferredoxin Ferredoxin NADP+-reductase Complex Docking and NMR
Contexto Educativo
Citação
Editora
Elsevier B.V. on behalf of the Federation of European Biochemical Societies