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PEDOT-Coated PLA Fibers Electrospun from Solutions Incorporating Fe(III)Tosylate in Different Solvents by Vapor-Phase Polymerization for Neural Regeneration
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Orientador(es)
Resumo(s)
Therapeutic solutions for injuries in the peripheral nervous system are limited and not existing in the case of the central nervous system. The electrical stimulation of cells through a cell-supporting conductive scaffold may contribute to new therapeutic solutions for nerve regeneration. In this work, biocompatible Polylactic acid (PLA) fibrous scaffolds incorporating Fe(III)Tosylate (FeTos) were produced by electrospinning a mixture of PLA/FeTos solutions towards a rotating cylinder, inducing fiber alignment. Fibers were coated with the conductive polymer Poly(3,4 ethylenedioxythiophene) (PEDOT) formed by vapor-phase polymerization of EDOT at 70 °C for 2 h. Different solvents (ETH, DMF and THF) were used as FeTos solvents to investigate the impact on the scaffold’s conductivity. Scaffold conductivity was estimated to be as high as 1.50 × 10−1 S/cm when FeTos was dissolved in DMF. In vitro tests were performed to evaluate possible scaffold cytotoxicity, following ISO 10993-5, revealing no cytotoxic effects. Differentiation and growth of cells from the neural cell line SH-SY5Y seeded on the scaffolds were also assessed, with neuritic extensions observed in cells differentiated in neurons with retinoic acid. These extensions tended to follow the preferential alignment of the scaffold fibers.
Descrição
This research was co-financed by FEDER, European funds, through the COMPETE 2020 POCI and PORL and National Funds through FCT, Portuguese Foundation for Science and Technology and POR Lisboa2020, under the projects PIDDAC (POCI-01-0145-FEDER-007688, reference UIDB/50025/2020–2023) and PTDC/CTMCOM/32606/2017 (NEURiTES).
Publisher Copyright:
© 2023 by the authors.
Palavras-chave
conductivity electrospinning Fe(III)Tosylate neural regeneration PEDOT SH-SY5Y neuronal growth vapor-phase polymerization General Chemistry Polymers and Plastics SDG 7 - Affordable and Clean Energy SDG 9 - Industry, Innovation, and Infrastructure SDG 12 - Responsible Consumption and Production