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Exploring substrate interaction in respiratory alternative complex III from Rhodothermus marinus
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Resumo(s)
Rhodothermus marinus is a thermohalophilic organism that has optimized its microaerobic metabolism at 65 °C. We have been exploring its respiratory chain and observed the existence of a quinone:cytochrome c oxidoreductase complex, named Alternative Complex III, structurally different from the bc1 complex. In the present work, we took profit from nanodiscs and liposomes technology to investigate ACIII activity in membrane-mimicking systems. In addition, we studied the interaction of ACIII with menaquinone, its potential electron acceptors (HiPIP and cytochrome c) and the caa3 oxygen reductase.
Descrição
Funding Information: The work was funded by Fundação para a Ciência e a Tecnologia ( PTDC/BIA-BQM/30528/2017 ). The project was further supported by UIDB/04046/2020 and UIDP/04046/2020 Centre grants from FCT , Portugal (to BioISI), by LISBOA-01-0145-FEDER-007660 cofunded by FEDER through COMPETE2020-POCI and by Fundac̨ão para a Ciȇncia e a Tecnologia and by UIDB/04612/2020 and UIDP/04612/2020 research unit grants from FCT (to Mostmicro). This work benefited from access to CERMAX, ITQB-NOVA, Oeiras, Portugal with equipment funded by FCT, project AAC 01/SAICT/2016. Publisher Copyright: © 2023 The Authors
Palavras-chave
Alternative complex III (ACIII) Cytochrome bc1 High Potential Iron Sulfur Protein (HiPIP) Menaquinone Respiratory chain Thermophile Biophysics Biochemistry Cell Biology