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Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals

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To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.

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INFANT-DEATH-SYNDROME F-SP HOMINIS GENERAL-POPULATION CARINII-PNEUMONIA JIROVECII DNA COLONIZATION INFECTION Microbiology (medical) Infectious Diseases SDG 3 - Good Health and Well-being

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