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Molecular determinants of nonaqueous biocatalysis
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Resumo(s)
Over the last thirty years, the tremendous biotechnological potential of
nonaqueous biocatalysis has boosted research efforts in this area. Numerous
studies have tried to elucidate how enzymes work in these nonconventional
media and many properties are now well understood. However, when this
thesis was initiated, some aspects of this field were poorly characterized at
the molecular level. In particular, the molecular determinants of protein-ion
interactions, enzyme stability, and molecular memory, are important issues
which were lacking a thorough molecular analysis. These three subjects are
herein investigated using molecular simulation methodologies.(...)
Descrição
Dissertation presented to obtain the Ph.D degree in Biochemistry
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Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica