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Post-transcriptional Control Strategies in Synthetic Biology
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| 116.95 MB | Adobe PDF |
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"The revolution of genomics and growth of systems biology urged
the creation of synthetic biology, an engineering discipline aiming at
recreating and reprogramming cellular functions for industrial needs.
There has been a large effort in synthetic biology to develop versatile
and programmable genetic regulators that would enable the precise
control of gene expression. Synthetic RNA components have
emerged as a solution, offering a diverse range of programmable
functions, including signal sensing, gene regulation and the
modulation of molecular interactions. Owing to their compactness,
structure and way of action, several types of RNA devices that act
on DNA, RNA and protein have been characterized and applied in
synthetic biology. RNA-based approaches are more ‘economical’ for
the cell, since they are generally not translated. These RNA-based
strategies act on a much shorter time scale than transcription-based
ones and can be more efficient than protein-based mechanisms. In
this PhD, we explored these RNA components as building blocks in
the RNA synthetic biology field. The components were tested in two
different model microorganisms, the widely studied and industrially
used bacterium, Escherichia coli, and one of the most stress
resistant and industrially promising bacteria, Pseudomonas putida.(...)"
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Palavras-chave
mRNA gene expression