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Publicação
Microfluidics-driven fabrication of a low cost and ultrasensitive SERS-based paper biosensor
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Orientador(es)
Resumo(s)
Surface-enhanced Raman scattering (SERS) spectroscopy stands out due to its sensitivity, selectivity, and multiplex ability. The development of ready-to-use, simple, and low-cost SERS substrates is one of the main challenges of the field. In this paper, the intrinsic reproducibility of microfluidics technology was used for the fabrication of self-assembled nanoparticle structures over a paper film. The paper SERS substrates were fabricated by assembling anisotropic particles, gold nanostars (GNSs), and nanorods (NRs) onto paper to offer an extra enhancement to reach ultra-sensitive detection limits. A polydimethylsiloxane PDMS-paper hybrid device was used to control the drying kinetics of the nanoparticles over the paper substrate. This method allowed a high reproducibility and homogeneity of the fabrication of SERS substrates that reach limits of detection down to the picomolar range. This simple and low-cost fabrication of a paper-based sensing device was tested for the discrimination of different cell lineages.
Descrição
funded by the "Innovative Microfluidic Platform for Analysis of myeloid Leukemia blasts" project (030782).
co-funded by FCT and the ERDF through COMPETE2020, the CANCER project (NORTE-01-0145-FEDER-000029) funded through the NORTE-45-2015-02 program.
Palavras-chave
Hybrid Microfluidics Nano-biosensors Nanoparticles Nanotechnology Self-assembly SERS General Materials Science Instrumentation General Engineering Process Chemistry and Technology Computer Science Applications Fluid Flow and Transfer Processes