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Aspergillus section fumigati in firefighter headquarters

2021-10Artigo científico Acesso aberto
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dc.contributor.authorViegas, Carla
dc.contributor.authorGomes, Bianca
dc.contributor.authorDias, Marta
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.institutionComprehensive Health Research Centre (CHRC) - Pólo ENSP
dc.contributor.institutionCentro de Investigação em Saúde Pública (CISP/PHRC)
dc.contributor.pblMDPI AG
dc.date.accessioned2021-10-22T03:43:32Z
dc.date.available2021-10-22T03:43:32Z
dc.date.issued2021-10
dc.descriptionFunding Information: This work was supported by Instituto Polit?cnico de Lisboa, for funding the Project ?Occu-pational exposure of ambulance drivers to bioburden? (IPL/2020/BIO-AmbuDrivers_ESTeSL)?. Funding Information: Funding: This work was supported by Instituto Politécnico de Lisboa, for funding the Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIO-AmbuDrivers_ESTeSL)”. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractBackground: Aspergillus section Fumigati is one of the Aspergillus sections more frequently related to respiratory symptoms and by other health outcomes. This study aimed to characterize Aspergillus section Fumigati distribution in eleven firefighter headquarters (FFHs) to obtain an accurate occupational exposure assessment. Methods: A sampling approach protocol was performed using active (impaction method) and passive sampling methods (floor surfaces swabs, electrostatic dust collectors (EDCs), and settled dust). All samples were analysed by culture-based methods and passive sampling was used for molecular detection of Aspergillus section Fumigati. Results: Of all the matrices, the highest counts of Aspergillus sp. were obtained on settled dust filters (3.37% malt extract agar—MEA, 19.09% dichloran glycerol—DG18) followed by cleaning cloths (1.67% MEA; 7.07% DG18). Among the Aspergillus genus, the Fumigati section was predominant in Millipore and EDC samples in MEA (79.77% and 28.57%, respectively), and in swabs and settled dust filters in DG18 (44.76% and 30%, respectively). The Fumigati section was detected more frequently in DG18 (33.01%) compared to MEA (0.33%). The Fumigati section was observed in azole supplemented media (itraconazole and voriconazole) in several passive sampling methods employed and detected by qPCR in almost all passive samples, with EDCs being the matrix with the highest prevalence (n = 61; 67.8%). Conclusion: This study confirms that Aspergillus sp. is widespread and the Fumigati section is present in all FFHs. The presence of fungi potentially resistant to azoles in the FFHs was also observed. Further studies are needed to identify the best corrective and preventive measures to avoid this section contamination in this specific occupational environment.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2232691
dc.identifier.doi10.3390/microorganisms9102112
dc.identifier.issn2076-2607
dc.identifier.otherPURE: 34340732
dc.identifier.otherPURE UUID: b02bf082-1354-4de3-95c0-2b8fa913e08a
dc.identifier.otherScopus: 85116449833
dc.identifier.otherORCID: /0000-0003-0809-8367/work/101664093
dc.identifier.otherPubMed: 34683433
dc.identifier.otherPubMedCentral: PMC8541501
dc.identifier.otherWOS: 000713156700001
dc.identifier.urihttp://hdl.handle.net/10362/126493
dc.identifier.urlhttps://www.scopus.com/pages/publications/85116449833
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAzole resistance profile
dc.subjectCulture-based methods
dc.subjectMolecular tools
dc.subjectSampling approach
dc.subjectMicrobiology
dc.subjectMicrobiology (medical)
dc.subjectVirology
dc.titleAspergillus section fumigati in firefighter headquartersen
dc.typejournal article
degois.publication.issue10
degois.publication.titleMicroorganisms
degois.publication.volume9
dspace.entity.typePublication
rcaap.rightsopenAccess

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