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Growth of Ehrlichia canis, the causative agent of canine monocytic ehrlichiosis, in vector and non-vector ixodid tick cell lines

2016Artigo científico Acesso aberto
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dc.contributor.authorFerrolho, J
dc.contributor.authorSimpson, Jennifer
dc.contributor.authorHawes, Philippa
dc.contributor.authorZweygarth, Erich
dc.contributor.authorBell-Sakyi, Lesley
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.pblElsevier
dc.date.accessioned2022-07-22T22:19:47Z
dc.date.available2022-07-24T00:31:41Z
dc.date.embargoedUntil2022-07-23
dc.date.issued2016
dc.description.abstractCanine monocytic ehrlichiosis is caused by Ehrlichia canis, a small gram-negative coccoid bacterium that infects circulating monocytes. The disease is transmitted by the brown dog tick Rhipicephalus sanguineus s.l. and is acknowledged as an important infectious disease of dogs and other members of the family Canidae worldwide. E. canis is routinely cultured in vitro in the canine monocyte-macrophage cell line DH82 and in non-vector Ixodes scapularis tick cell lines, but not in cells derived from its natural vector. Here we report infection and limited propagation of E. canis in the tick cell line RSE8 derived from the vector R. sanguineus s.l., and successful propagation through six passages in a cell line derived from the experimental vector Dermacentor variabilis. In addition, using bacteria semi-purified from I. scapularis cells we attempted to infect a panel of cell lines derived from non-vector species of the tick genera Amblyomma, Dermacentor, Hyalomma, Ixodes and Rhipicephalus with E. canis and, for comparison, the closely-related Ehrlichia ruminantium, causative agent of heartwater in ruminants. Amblyomma and non-vector Dermacentor spp. cell lines appeared refractory to infection with E. canis but supported growth of E. ruminantium, while some, but not all, cell lines derived from Hyalomma, Ixodes and Rhipicephalus spp. ticks supported growth of both pathogens. We also illustrated and compared the ultrastructural morphology of E. canis in DH82, RSE8 and I. scapularis IDE8 cells. This study confirms that E. canis, like E. ruminantium, is able to grow not only in cell lines derived from natural and experimental tick vectors but also in a wide range of other cell lines derived from tick species not known to transmit this pathogen.en
dc.description.versionauthorsversion
dc.description.versionpublished
dc.format.extent7
dc.format.extent1177088
dc.identifier.doi10.1016/j.ttbdis.2016.01.013
dc.identifier.issn1877-959X
dc.identifier.otherPURE: 2450460
dc.identifier.otherPURE UUID: 6107c80e-98e8-40ce-b58c-e6c1e778c333
dc.identifier.otherScopus: 84961777303
dc.identifier.otherWOS: 000379279000011
dc.identifier.otherPubMed: 26837859
dc.identifier.urihttp://hdl.handle.net/10362/142321
dc.identifier.urlhttps://www.scopus.com/pages/publications/84961777303
dc.language.isoeng
dc.peerreviewedyes
dc.subjectDermacentor variabilis
dc.subjectEhrlichia canis
dc.subjectEhrlichia ruminantium
dc.subjectElectron microscopy
dc.subjectRhipicephalus sanguineus s.l.
dc.subjectTick cell lines
dc.subjectInfectious Diseases
dc.subjectInsect Science
dc.subjectParasitology
dc.subjectMicrobiology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleGrowth of Ehrlichia canis, the causative agent of canine monocytic ehrlichiosis, in vector and non-vector ixodid tick cell linesen
dc.typejournal article
degois.publication.firstPage631
degois.publication.issue4
degois.publication.lastPage637
degois.publication.titleTicks and Tick-borne Diseases
degois.publication.volume7
dspace.entity.typePublication
rcaap.rightsopenAccess

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