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Modulation of human phenylalanine hydroxylase by 3-hydroxyquinolin-2(1h)-one derivatives

2021-03Artigo científico Acesso aberto
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dc.contributor.authorLopes, Raquel R.
dc.contributor.authorTomé, Catarina S.
dc.contributor.authorRusso, Roberto
dc.contributor.authorPaterna, Roberta
dc.contributor.authorLeandro, João
dc.contributor.authorCandeias, Nuno R.
dc.contributor.authorGonçalves, Lídia M.D.
dc.contributor.authorTeixeira, Miguel
dc.contributor.authorSousa, Pedro M.F.
dc.contributor.authorGuedes, Rita C.
dc.contributor.authorVicente, João B.
dc.contributor.authorGois, Pedro M.P.
dc.contributor.authorLeandro, Paula
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-07-09T22:18:00Z
dc.date.available2021-07-09T22:18:00Z
dc.date.issued2021-03
dc.descriptionFunding Information: We are grateful to Cristina Leit?o (ITQB-NOVA) for expert technical assistance on the HPLC assays. We thank the Diamond Light Source for access to beamline B21 (proposal mx20161) and the technical support from Katsuaki Inoue, that contributed to the results presented here.This work was supported by FEDER and Funda??o para a Ci?ncia e a Tecnologia, I. P. through national funds (Projects UIDB/04138/2020 and UIDP/04138/2020 and research project PTDC/MED-QUI/29712/2017). This work has received funding from the European Union?s Horizon 2020 research and innovation programme under the Marie-Sklodowska Curie grant agreement No [675007], LISBOA-01-0145-FEDER-029967 and PTDC/QUI-QOR/29967/2017. Principal Researcher grant CEECIND/03143/2017 (Funda??o para a Ci?ncia e a Tecnologia) is acknowledged by L.M.D.G. European Union?s Horizon 2020 research and innovation programme (grant agreement No 810856) is acknowledged by M.T. iNOVA4Health Research Unit (LISBOA-01-0145-FEDER-007344), which is cofunded by Funda??o para a Ci?ncia e a Tecnologia/Minist?rio da Ci?ncia, Tecnologia e Ensino Superior, through national funds, and by FEDER under the PT2020 Partnership Agreement, is acknowledged by J.B.V. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
dc.description.abstractPhenylketonuria (PKU) is a genetic disease caused by deficient activity of human pheny-lalanine hydroxylase (hPAH) that, when untreated, can lead to severe psychomotor impairment. Protein misfolding is recognized as the main underlying pathogenic mechanism of PKU. Therefore, the use of stabilizers of protein structure and/or activity is an attractive therapeutic strategy for this condition. Here, we report that 3-hydroxyquinolin-2(1H)-one derivatives can act as protectors of hPAH enzyme activity. Electron paramagnetic resonance spectroscopy demonstrated that the 3-hydroxyquinolin-2(1H)-one compounds affect the coordination of the non-heme ferric center at the enzyme active-site. Moreover, surface plasmon resonance studies showed that these stabilizing compounds can be outcompeted by the natural substrate L-phenylalanine. Two of the designed compounds functionally stabilized hPAH by maintaining protein activity. This effect was observed on the recombinant purified protein and in a cellular model. Besides interacting with the catalytic iron, one of the compounds also binds to the N-terminal regulatory domain, although to a different location from the allosteric L-Phe binding site, as supported by the solution structures obtained by small-angle X-ray scattering.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent25
dc.format.extent48517266
dc.identifier.doi10.3390/biom11030462
dc.identifier.issn2218-273X
dc.identifier.otherPURE: 32441039
dc.identifier.otherPURE UUID: 729f9ed4-3c3a-4718-94fd-0b0df7bb469e
dc.identifier.otherScopus: 85102718258
dc.identifier.otherPubMed: 33808760
dc.identifier.urihttp://hdl.handle.net/10362/120790
dc.identifier.urlhttps://www.scopus.com/pages/publications/85102718258
dc.language.isoeng
dc.peerreviewedyes
dc.subjectActivity chaperones
dc.subjectDrug discovery
dc.subjectInherited metabolic disorders
dc.subjectPharmacological chaperones
dc.subjectProtein drug interactions
dc.subjectProtein misfolding
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleModulation of human phenylalanine hydroxylase by 3-hydroxyquinolin-2(1h)-one derivativesen
dc.typejournal article
degois.publication.firstPage1
degois.publication.issue3
degois.publication.lastPage25
degois.publication.titleBiomolecules
degois.publication.volume11
dspace.entity.typePublication
rcaap.rightsopenAccess

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