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Metabolic pathway for propionate utilization by phosphorus-accumulating organisms in activated sludge: 13C labeling and in vivo nuclear magnetic resonance

2003-01-01Artigo científico Acesso aberto
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dc.contributor.authorLemos, Paulo C.
dc.contributor.authorSerafim, Luísa S.
dc.contributor.authorSantos, Margarida M.
dc.contributor.authorReis, Maria A. M.
dc.contributor.authorSantos, Helena
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.institutionMolecular, Structural and Cellular Microbiology (MOSTMICRO)
dc.contributor.pblAmerican Society for Microbiology
dc.date.accessioned2019-03-08T23:18:29Z
dc.date.available2019-03-08T23:18:29Z
dc.date.issued2003-01-01
dc.description.abstractIn vivo 13C and 31P nuclear magnetic resonance techniques were used to study propionate metabolism by activated sludge in enhanced biological phosphorus removal systems. The fate of label supplied in [3-13C] propionate was monitored in living cells subjected to anaerobic/aerobic cycles. During the anaerobic phase, propionate was converted to polyhydroxyalkanoates (PHA) with the following monomer composition: hydroxyvalerate, 74.2%; hydroxymethylvalerate, 16.9%; hydroxymethylbutyrate, 8.6%; and hydroxybutyrate, 0.3%. The isotopic enrichment in the different carbon atoms of hydroxyvalerate (HV) produced during the first anaerobic stage was determined: HV5, 59%; HV4, 5.0%; HV3, 1.1%; HV2, 3.5%; and HV1, 2.8%. A large proportion of the supplied label ended up on carbon C-5 of HV, directly derived from the pool of propionyl-coenzyme A (CoA), which is primarily labeled on C-3; useful information on the nature of operating metabolic pathways was provided by the extent of labeling on C-1, C-2, and C-4. The labeling pattern on C-1 and C-2 was explained by the conversion of proplonyl-CoA to acetyl-CoA via succinyl-CoA and the left branch of the tricarboxylic acid cycle, which involves scrambling of label between the inner carbons of succinate. This constitutes solid evidence for the operation of succinate dehydrogenase under anaerobic conditions. The labeling in HV4 is explained by backflux from succinate to propionyl-CoA. The involvement of glycogen in the metabolism of propionate was also demonstrated; moreover, it was shown that the acetyl moiety to the synthesis of PHA was derived preferentially from glycogen. According to the proposed metabolic scheme, the decarboxylation of pyruvate is coupled to the production of hydrogen, and the missing reducing equivalents should be derived from a source other than glycogen metabolism.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent11
dc.format.extent291485
dc.identifier.doi10.1128/AEM.69.1.241-251.2003
dc.identifier.issn0099-2240
dc.identifier.otherPURE: 11937886
dc.identifier.otherPURE UUID: 3799b0f0-aa67-4f40-ae46-dcb106c77aa9
dc.identifier.otherScopus: 0348209610
dc.identifier.otherPubMed: 12514001
dc.identifier.otherWOS: 000180328000032
dc.identifier.otherPubMedCentral: PMC152432
dc.identifier.otherORCID: /0000-0001-6094-0107/work/64041997
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=0348209610&partnerID=8YFLogxK
dc.identifier.urlhttps://www.scopus.com/pages/publications/0348209610
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBiotechnology
dc.subjectFood Science
dc.subjectApplied Microbiology and Biotechnology
dc.subjectEcology
dc.titleMetabolic pathway for propionate utilization by phosphorus-accumulating organisms in activated sludge: 13C labeling and in vivo nuclear magnetic resonanceen
dc.typejournal article
degois.publication.firstPage241
degois.publication.issue1
degois.publication.lastPage251
degois.publication.titleApplied and Environmental Microbiology
degois.publication.volume69
dspace.entity.typePublication
rcaap.rightsopenAccess

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