Logo do repositório
 
Publicação

Development of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samples

2021-06Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorFilipa-Silva, Andreia
dc.contributor.authorNunes, Mónica
dc.contributor.authorParreira, Ricardo
dc.contributor.authorCrespo, Maria Teresa Barreto
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.pblMDPI AG
dc.date.accessioned2021-06-14T22:16:18Z
dc.date.available2021-06-14T22:16:18Z
dc.date.issued2021-06
dc.descriptionFunding Information: from Multi Biorefinery project (POCI-01?0145-FEDER-016403, INTERFACE Program, through the Innovation, Technology and Circular Economy Fund (FITEC) (CIT/2018/15) and FCT for Andreia Silva fellowship (PD/BD/128363/2017) are gratefully acknowledged. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
dc.description.abstractHuman enteric viruses such as norovirus (NoV) and hepatitis A virus (HAV) are some of the most important causes of foodborne infections worldwide. Usually, infection via fish consumption is not a concern regarding these viruses, since fish are mainly consumed cooked. However, in the last years, raw fish consumption has become increasingly common, especially involving the use of seabass and gilthead seabream in dishes like sushi, sashimi, poke, and carpaccio. Therefore, the risk for viral infection via the consumption of raw fish has also increased. In this study, a virologic screening was performed in 323 fish specimens captured along the Portuguese coast using a tetraplex qPCR optimised for two templates (plasmid and in vitro transcribed RNA) to detect and quantify NoV GI, NoV GII and HAV genomes. A difference of approximately 1-log was found between the use of plasmid or in vitro transcribed RNA for molecular-based quantifications, showing an underestimation of genome copy-number equivalents using plasmid standard-based curves. Additionally, the presence of NoV genomic RNA in a pool of seabass brains was identified, which was shown to cluster with a major group of human norovirus sequences from genogroup I (GI.1) by phylogenetic analysis. None of the analysed fish revealed the presence of NoV GII or HAV. This result corroborates the hypothesis that enteric viruses circulate in seawater or that fish were contaminated during their transportation/handling, representing a potential risk to humans through raw or undercooked fish consumption.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1526122
dc.identifier.doi10.3390/microorganisms9061149
dc.identifier.issn2076-2607
dc.identifier.otherPURE: 31821147
dc.identifier.otherPURE UUID: 19183fb7-6f0d-47b9-8140-ba138ddf5a65
dc.identifier.otherScopus: 85106615088
dc.identifier.otherORCID: /0000-0002-4832-9898/work/95136498
dc.identifier.urihttp://hdl.handle.net/10362/119238
dc.identifier.urlhttps://www.scopus.com/pages/publications/85106615088
dc.language.isoeng
dc.peerreviewedyes
dc.subjectFish
dc.subjectHepatitis A
dc.subjectNorovirus
dc.subjectPathogenic human viruses
dc.subjectTetraplex qPCR assay
dc.subjectMicrobiology
dc.subjectMicrobiology (medical)
dc.subjectVirology
dc.subjectMolecular Biology
dc.subjectSDG 3 - Good Health and Well-being
dc.subjectSDG 12 - Responsible Consumption and Production
dc.subjectSDG 14 - Life Below Water
dc.titleDevelopment of a tetraplex qpcr for the molecular identification and quantification of human enteric viruses, nov and hav, in fish samplesen
dc.typejournal article
degois.publication.issue6
degois.publication.titleMicroorganisms
degois.publication.volume9
dspace.entity.typePublication
rcaap.rightsopenAccess

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Development_of_a_tetraplex_qpcr_for_the_molecular_identification_and_quantification_of_human_enteric_viruses_nov_and_hav_in_fish_samples.pdf
Tamanho:
1.46 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

IHMT: MM - Artigos em revista internacional com arbitragem científica