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Early arthritis induces disturbances at bone nanostructural level reflected in decreased tissue hardness in an animal model of arthritis

2018-01Artigo científico Acesso aberto
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dc.contributor.authorVidal, Bruno
dc.contributor.authorCascão, Rita
dc.contributor.authorFinnilä, Mikko A.J.
dc.contributor.authorLopes, Inês P.
dc.contributor.authorSaarakkala, Simo
dc.contributor.authorZioupos, Peter
dc.contributor.authorCanhão, Helena
dc.contributor.authorCanhão, Helena
dc.contributor.authorFonseca, João E.
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.pblPLOS - Public Library of Science
dc.date.accessioned2018-01-23T23:15:25Z
dc.date.available2018-01-23T23:15:25Z
dc.date.issued2018-01
dc.description.abstractIntroduction Arthritis induces joint erosions and skeletal bone fragility. Objectives The main goal of this work was to analyze the early arthritis induced events at bone architecture and mechanical properties at tissue level. Methods Eighty-eight Wistar rats were randomly housed in experimental groups, as follows: adjuvant induced arthritis (AIA) (N = 47) and a control healthy group (N = 41). Rats were monitored during 22 days for the inflammatory score, ankle perimeter and body weight and sacrificed at different time points (11 and 22 days post disease induction). Bone samples were collected for histology, micro computed tomography (micro-CT), 3-point bending and nanoindentation. Blood samples were also collected for bone turnover markers and systemic cytokine quantification. Results At bone tissue level, measured by nanoindentation, there was a reduction of hardness in the arthritic group, associated with an increase of the ratio of bone concentric to parallel lamellae and of the area of the osteocyte lacuna. In addition, increased bone turnover and changes in the microstructure and mechanical properties were observed in arthritic animals, since the early phase of arthritis, when compared with healthy controls. Conclusion We have shown in an AIA rat model that arthritis induces very early changes at bone turnover, structural degradation and mechanical weakness. Bone tissue level is also affected since the early phase of arthritis, characterized by decreased tissue hardness associated with changes in bone lamella organization and osteocyte lacuna surface. These observations highlight the pertinence of immediate control of inflammation in the initial stages of arthritis.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent562377
dc.identifier.doi10.1371/journal.pone.0190920
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 3537810
dc.identifier.otherPURE UUID: d8196795-5bdb-40ad-a686-6792f708ae4e
dc.identifier.otherScopus: 85040231054
dc.identifier.otherWOS: 000419655900033
dc.identifier.otherPubMed: 29315314
dc.identifier.otherPubMedCentral: PMC5760022
dc.identifier.otherORCID: /0000-0003-1894-4870/work/67178087
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=85040231054&partnerID=8YFLogxK
dc.identifier.urlhttps://www.scopus.com/pages/publications/85040231054
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.subjectGeneral Agricultural and Biological Sciences
dc.titleEarly arthritis induces disturbances at bone nanostructural level reflected in decreased tissue hardness in an animal model of arthritisen
dc.typejournal article
degois.publication.issue1
degois.publication.titlePLoS ONE
degois.publication.volume13
dspace.entity.typePublication
person.familyNameCanhão
person.givenNameHelena
person.identifier379800
person.identifier.ciencia-id5A17-C6D9-DBC8
person.identifier.orcid0000-0003-1894-4870
person.identifier.ridC-9611-2018
person.identifier.scopus-author-id6602393492
rcaap.rightsopenAccess
relation.isAuthorOfPublicationc87f58c0-b53d-4321-9bd7-39554e6001b5
relation.isAuthorOfPublication.latestForDiscoveryc87f58c0-b53d-4321-9bd7-39554e6001b5

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