Logo do repositório
 
Publicação

Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?

2020-11Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorCarvalho, Ana Sofia
dc.contributor.authorMoraes, Maria Carolina Strano
dc.contributor.authorNa, Chan Hyun
dc.contributor.authorFierro-Monti, Ivo
dc.contributor.authorHenriques, Andreia
dc.contributor.authorZahedi, Sara
dc.contributor.authorZahedi, Sara
dc.contributor.authorBodo, Cristian
dc.contributor.authorTranfield, Erin M.
dc.contributor.authorSousa, Ana Laura
dc.contributor.authorFarinho, Ana
dc.contributor.authorRodrigues, Luís Vaz
dc.contributor.authorPinto, Paula
dc.contributor.authorBárbara, Cristina
dc.contributor.authorMota, Leonor
dc.contributor.authorde Abreu, Tiago Tavares
dc.contributor.authorSemedo, Júlio
dc.contributor.authorSeixas, Susana
dc.contributor.authorKumar, Prashant
dc.contributor.authorCosta-Silva, Bruno
dc.contributor.authorPandey, Akhilesh
dc.contributor.authorMatthiesen, Rune
dc.contributor.authorMatthiesen, Rune
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.institutioniNOVA4Health - pólo NMS
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2020-12-30T05:04:00Z
dc.date.available2020-12-30T05:04:00Z
dc.date.issued2020-11
dc.descriptionFunding: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UID/Multi/04462/2013), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. This work was supported by the Wellcome Trust/DBT India Alliance Margdarshi Fellowship (grant number IA/M/15/1/502023) awarded to A.P. B.C.-S., M.C.S.C. and C.B. are supported by the Champalimaud Foundation and the EMBO Installation Grant 3921.
dc.description.abstractAcellular bronchoalveolar lavage (BAL) proteomics can partially separate lung cancer from non-lung cancer patients based on principal component analysis and multivariate analysis. Furthermore, the variance in the proteomics data sets is correlated mainly with lung cancer status and, to a lesser extent, smoking status and gender. Despite these advances BAL small and large extracellular vehicles (EVs) proteomes reveal aberrant protein expression in paracrine signaling mechanisms in cancer initiation and progression. We consequently present a case-control study of 24 bronchoalveolar lavage extracellular vesicle samples which were analyzed by state-of-the-art liquid chromatography-mass spectrometry (LC-MS). We obtained evidence that BAL EVs proteome complexity correlated with lung cancer stage 4 and mortality within two years´ follow-up (p value = 0.006). The potential therapeutic target DNMT3B complex is significantly up-regulated in tumor tissue and BAL EVs. The computational analysis of the immune and fibroblast cell markers in EVs suggests that patients who deceased within the follow-up period display higher marker expression indicative of innate immune and fibroblast cells (four out of five cases). This study provides insights into the proteome content of BAL EVs and their correlation to clinical outcomes.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent18
dc.format.extent3442762
dc.identifier.doi10.3390/cancers12113450
dc.identifier.issn2072-6694
dc.identifier.otherPURE: 26662759
dc.identifier.otherPURE UUID: 3c76aaf7-6c27-443f-a3b4-417c5ff48c62
dc.identifier.otherScopus: 85096429584
dc.identifier.otherWOS: 000592900800001
dc.identifier.otherPubMed: 33233545
dc.identifier.urihttp://hdl.handle.net/10362/109483
dc.identifier.urlhttps://www.scopus.com/pages/publications/85096429584
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBronchoalveolar lavage
dc.subjectExtracellular vesicles
dc.subjectImmuno oncology
dc.subjectLung cancer
dc.subjectProteomics
dc.subjectOncology
dc.subjectCancer Research
dc.subjectSDG 3 - Good Health and Well-being
dc.titleIs the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?en
dc.typejournal article
degois.publication.firstPage1
degois.publication.issue11
degois.publication.lastPage18
degois.publication.titleCancers
degois.publication.volume12
dspace.entity.typePublication
person.familyNameZahedi
person.familyNameMatthiesen
person.givenNameSara
person.givenNameRune
person.identifier1319342
person.identifier.ciencia-id7B1A-CEAC-23D9
person.identifier.ciencia-idBB1A-A606-EE77
person.identifier.orcid0000-0001-8199-9394
person.identifier.orcid0000-0002-6353-2616
person.identifier.scopus-author-id9741897800
rcaap.rightsopenAccess
relation.isAuthorOfPublicationb0d1f909-e648-4976-b61f-8acd3501d4c4
relation.isAuthorOfPublication67181d3c-b83e-4151-9a74-0ea14aeb7f05
relation.isAuthorOfPublication.latestForDiscoveryb0d1f909-e648-4976-b61f-8acd3501d4c4

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
cancers_12_03450_v2.pdf
Tamanho:
3.28 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

NMS: iNOVA4Health - Artigos em revista internacional com arbitragem científica