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Bioprocess engineering as a tool to modulate the rheological performance of Ensifer meliloti SEMIA 135 exopolysaccharide

2026-04Artigo científico Acesso aberto
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dc.contributor.authorFerreira Filho, Rui dos Santos
dc.contributor.authorFreitas, Filomena
dc.contributor.authorBurkert, Janaína Fernandes de Medeiros
dc.contributor.authorBurkert, Carlos André Veiga
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.pblElsevier
dc.date.accessioned2026-07-29T08:47:01Z
dc.date.available2026-07-29T08:47:01Z
dc.date.issued2026-04
dc.descriptionThis work was supported by FAPERGS (Foundation Research Support in the State of Rio Grande do Sul) (Grant Number 24/2551–0001776-3) and the Coordination for the Improvement of Higher Education Personnel (CAPES) (Finance Code 001). Publisher Copyright: © 2026 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license. http://creativecommons.org/licenses/by/4.0/
dc.description.abstractThe controlled production of microbial polysaccharides offers a powerful route to design renewable materials with tailored functionalities. In this work, the influence of bioreactor hydrodynamics on the structure and macroscopic properties of the exopolysaccharide produced by Ensifer meliloti SEMIA 135 was systematically investigated. Aeration (0.8–2.2 vvm) and agitation (59–341 rpm) were varied according to a Central Composite Rotatable Design, revealing a strong interaction effect on polymer composition and viscosity. Variations in cultivation conditions modulated the apparent viscosity from 68.7 to 228.3 Pa·s, correlated with a variation in total uronic acid content from 2.91% to 11.96%, confirming the key role of charge density in defining molecular organization. The polymer formed weak, pseudoplastic, and thixotropic gels, with the storage modulus (G′) consistently higher than the loss modulus (G″) across all frequencies. Its viscoelastic profile was highly dependent on concentration (0.01–3.0 wt%), pH (3–9), and temperature, showing a sol–gel transition near 70 °C and enhanced elasticity at acidic and alkaline pH. Deviations from the Cox–Merz rule indicated the presence of a structured associative network rather than a simple entangled solution. Overall, this study demonstrates that fine-tuning of cultivation conditions can be used as a predictive tool to control the molecular architecture and rheological performance of bacterial polysaccharides, establishing a rational bioprocessing framework for the development of functional biomaterials.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent15
dc.format.extent7623755
dc.identifier.doi10.1016/j.ijbiomac.2026.151590
dc.identifier.issn0141-8130
dc.identifier.otherPURE: 169896261
dc.identifier.otherPURE UUID: a45c6fa6-e835-42a5-8995-bd6ed609e4ef
dc.identifier.otherScopus: 105034622028
dc.identifier.otherPubMed: 41871762
dc.identifier.otherORCID: /0000-0002-9430-4640/work/222228678
dc.identifier.urihttp://hdl.handle.net/10362/204929
dc.identifier.urlhttps://www.scopus.com/pages/publications/105034622028
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F04378%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F04378%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT
dc.subjectBiopolymers
dc.subjectBioreactor hydrodynamics
dc.subjectDiazotrophic bacteria
dc.subjectGelation
dc.subjectThixotropy
dc.subjectViscoelasticity
dc.subjectFood Science
dc.subjectStructural Biology
dc.subjectBiochemistry
dc.subjectBiomaterials
dc.subjectMolecular Biology
dc.subjectSDG 7 - Affordable and Clean Energy
dc.titleBioprocess engineering as a tool to modulate the rheological performance of Ensifer meliloti SEMIA 135 exopolysaccharideen
dc.typejournal article
degois.publication.titleInternational Journal of Biological Macromolecules
degois.publication.volume356
dspace.entity.typePublication
rcaap.rightsopenAccess

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