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A new approach for Chrysoporthe cubensis cellulolytic cocktail production using solid and submerged-state fermentation

2023-06Artigo científico Acesso aberto
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dc.contributor.authorDutra, Thiago Rodrigues
dc.contributor.authorDutra, Thiago Rodrigues
dc.contributor.authorGuimarães, Valéria Monteze
dc.contributor.authorVarela, Ednilson Mascarenhas
dc.contributor.authorde Albuquerque, Mariana Furtado Granato
dc.contributor.authorDíez, Juan Diego Rios
dc.contributor.authorAlfenas, Acelino Couto
dc.contributor.authorde Rezende, Sebastião Tavares
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblBrazilian Society of Chemical engineering
dc.date.accessioned2023-11-28T22:37:54Z
dc.date.available2023-11-28T22:37:54Z
dc.date.issued2023-06
dc.descriptionFunding Information: We thank Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico (CNPq) for the financial support and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for providing scholarships. We would like to thank Thamires Felisberto Pereira Dutra for cooperating in the experiments execution. Publisher Copyright: © 2023, The Author(s) under exclusive licence to Associação Brasileira de Engenharia Química.
dc.description.abstractThe lignocellulosic material bioconversion to bioproducts has received significant attention in recent years. Cellulases and hemicellulases catalyze the hydrolysis of lignocellulosic materials into fermentable sugars that are afterward converted to bioproducts by microorganisms. Chrysoporthe cubensis grown under solid-state fermentation (SSF) has produced more effective enzymatic extracts for sugarcane bagasse saccharification than commercial cellulolytic preparations. However, the investigation of new approaches for enzyme production by this fungus is still lacking. In this work, an enzyme cocktail (SSF-SmF-cocktail) was produced by extracting enzymes of C. cubensis grown under SSF using the extract produced by the same fungus under submerged fermentation (SmF). The total cellulase (FPase), carboxymethylcellulase (CMCase), cellobiohydrolase (CBH), β-glucosidase, xylanase, β-xylosidase, β-galactosidase, α-galactosidase, and α-arabinofuranosidase activities were evaluated in crude extracts obtained from C. cubensis cultivation under SSF, SmF, and also in the SSF-SmF-cocktail. The C. cubensis protein profiles cultivated under SSF and SmF were compared by SDS-PAGE. Extract produced by C. cubensis grown under SmF presented proteins with estimated molecular weights of 10.7, 29.3, 38.6, 46.0, and 170.0 kDa, respectively, but not in that produced by this fungus under SSF. When cultivated under SSF, C. cubensis produced an extract with greater protein diversity between 13 and 51 kDa than that obtained by this fungus under SmF. The 83.0 and 95.3 kDa protein bands were present in both C. cubensis cultures. The C. cubensis SSF-SmF-cocktail presented better efficiency in glucose release after 48 h of the alkali-pretreated sugarcane bagasse hydrolysis when compared to those produced by this fungus under either SSF or SmF. This extract showed the highest xylananase/FPase rate and the second highest CMCase/FPase and b-glucosidase/FPase rates among the evaluated extracts, suggesting that these enzymes are the main determinants of this cocktail the efficiency on the alkali pretreated sugarcane bagasse sacchariffication process. These results demonstrated that the enzymes produced by C. cubensis cultivated under SSF and SmF are complementary for the alkali-pretreated sugarcane bagasse enzymatic hydrolysis, since the SSF-SmF-cocktail was more efficient than other extracts produced by this fungus and that the commercial Accellerase®. Therefore, the SSF-SmF-cocktail is a promising alternative for industrial applications. Graphical abstract: [Figure not available: see fulltext.].en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent8
dc.format.extent965292
dc.identifier.doi10.1007/s43153-023-00309-y
dc.identifier.issn0104-6632
dc.identifier.otherPURE: 76753020
dc.identifier.otherPURE UUID: 0826e89a-39d5-4d9c-b3cf-8a38dfd99e74
dc.identifier.otherScopus: 85150522331
dc.identifier.urihttp://hdl.handle.net/10362/160676
dc.identifier.urlhttps://www.scopus.com/pages/publications/85150522331
dc.language.isoeng
dc.peerreviewedyes
dc.subjectChrysoporthe cubensis
dc.subjectSaccharification
dc.subjectSolid-state fermentation
dc.subjectSubmerged fermentation
dc.subjectGeneral Chemical Engineering
dc.titleA new approach for Chrysoporthe cubensis cellulolytic cocktail production using solid and submerged-state fermentationen
dc.typejournal article
degois.publication.firstPage359
degois.publication.issue2
degois.publication.lastPage366
degois.publication.titleBrazilian Journal Of Chemical Engineering
degois.publication.volume40
dspace.entity.typePublication
person.familyNameDutra
person.givenNameThiago Rodrigues
person.identifier.orcid0000-0003-4345-1621
rcaap.rightsopenAccess
relation.isAuthorOfPublication3e528e74-c1c0-41d1-966c-f6b0671a4d19
relation.isAuthorOfPublication.latestForDiscovery3e528e74-c1c0-41d1-966c-f6b0671a4d19

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