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Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis

2019-04Artigo científico Acesso aberto
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dc.contributor.authorRennerfeldt, Deena A.
dc.contributor.authorRaminhos, Joana S.
dc.contributor.authorLeff, Samantha M.
dc.contributor.authorManning, Pristinavae
dc.contributor.authorVan Vliet, Krystyn J.
dc.contributor.institutionDCM - Departamento de Ciência dos Materiais
dc.contributor.pblPLOS - Public Library of Science
dc.date.accessioned2020-09-25T22:21:58Z
dc.date.available2020-09-25T22:21:58Z
dc.date.issued2019-04
dc.descriptionGrant No. 1122374
dc.description.abstractBone marrow stromal cells (BMSCs) include a subset of stem cells that are considered promising for developmental studies and therapeutic applications. While it is appreciated generally that BMSC populations can exhibit morphological and functional heterogeneity upon in vitro culture expansion, the potential for heterogeneity within a single colony forming unit–generated ostensibly from a single mother cell–is less explored but is critical to design of both fundamental studies and cell therapy production. Here we observed BMSC colony formation in real time via time lapsed optical imaging and analysis, to quantify whether and how heterogeneity emerged over multiple cell divisions spanning the duration of a typical colony formation unit assay. These analyses demonstrate that such colonies are neither homogeneous subpopulations of stem cells nor necessarily derived from single originating cells. While the mechanisms for and causes of this intracolony heterogeneity are not understood fully, we further demonstrate that extensive cell-cell contacts do not correlate with senescence, but that media exchange was concurrent with diversification in even the most uniform single-cell-derived colonies. These direct quantitative observations and visualizations of colony formation provide new insights that are motivated by significant implications for both basic research and stem cell-based therapies.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent4685805
dc.identifier.doi10.1371/journal.pone.0213452
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 18764409
dc.identifier.otherPURE UUID: 1565c68e-fe25-4235-9156-5c092a808ce0
dc.identifier.otherScopus: 85063778103
dc.identifier.otherPubMed: 30943212
dc.identifier.otherPubMedCentral: PMC6447157
dc.identifier.otherWOS: 000463194300013
dc.identifier.urihttp://hdl.handle.net/10362/104744
dc.identifier.urlhttps://www.scopus.com/pages/publications/85063778103
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.subjectGeneral Agricultural and Biological Sciences
dc.subjectGeneral
dc.titleEmergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysisen
dc.typejournal article
degois.publication.issue4
degois.publication.titlePLoS ONE
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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