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In Vivo Antimicrobial Activity of Nisin Z Against S. aureus and Polyurea Pharmadendrimer PUREG4OEI48 Against P. aeruginosa from Diabetic Foot Infections

2025-05Artigo científico Acesso aberto
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dc.contributor.authorSerrano, Isa
dc.contributor.authorMil-Homens, Dalila
dc.contributor.authorPires, Rita F.
dc.contributor.authorBonifácio, Vasco D. B.
dc.contributor.authorGuerreiro, Joana F.
dc.contributor.authorCunha, Eva
dc.contributor.authorCosta, Sofia S.
dc.contributor.authorTavares, Luís
dc.contributor.authorOliveira, Manuela
dc.contributor.institutionLaboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionTB, HIV and opportunistic diseases and pathogens (THOP)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2025-09-28T21:42:04Z
dc.date.available2025-09-28T21:42:04Z
dc.date.issued2025-05
dc.descriptionFunding Information: This research was funded by national funds from the Fundação para a Ciência e a Tecnologia (FCT) (Projects PTDC/SAUINF/28466/2017 and PTDC/MEC-ONC/29327/2017); by the FCT in the scope of CIISA–Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa (Project UIDB/00276/2020), and Laboratório Associado para Ciência Animal e Veterinária (LA/P/0059/2020—AL4AnimalS); by the FCT in the scope of the Research Unit Institute for Bioengineering and Biosciences (iBB) (projects UIDB/04565/2020 and UIDP/04565/2020), and the Associate Laboratory Institute for Health and Bioeconomy (i4HB) (project LA/P/0140/2020); by the FCT in the scope of the Global Health and Tropical Medicine (GHTM) (project UID/04413/2020), and the Associate Laboratory in Translation and Innovation Towards Global Health (LA-REAL) (LA/P/0117/2020). Publisher Copyright: © 2025 by the authors.
dc.description.abstractBackground/Objectives: Diabetic foot infections (DFIs) are commonly associated with frequent hospitalizations, limb amputations, and premature death due to the profile of the bacteria infecting foot ulcers. DFIs are generally colonized by a polymicrobial net of bacteria that grows in biofilms, developing an increased antimicrobial resistance to multiple antibiotics. DFI treatment is a hurdle, and the need to develop new therapies that do not promote resistance is urgent. Therefore, the antibacterial efficacy of Nisin Z (antimicrobial peptide), a core–shell polycationic polyurea pharmadendrimer (PUREG4OEI48) (antimicrobial polymer), and amlodipine (antihypertensive drug) was evaluated against S. aureus and P. aeruginosa isolated from a DFI and previously characterized. Methods: The antibacterial activity was analyzed in vitro by determining the minimal inhibitory concentration (MIC) and in vivo in a Galleria mellonella model by assessing the larvae survival and health index. Results: The results indicate that Nisin Z exhibited antibacterial activity against S. aureus in vivo, allowing larvae full survival, and no antibacterial activity against P. aeruginosa. Nisin Z may have reduced the antibacterial effectiveness of both PUREG4OEI48 and amlodipine. PUREG4OEI48 significantly increased the survival of the larvae infected with P. aeruginosa, while amlodipine showed no activity against both bacteria in vivo. Conclusions: These findings suggest that both Nisin Z and PUREG4OEI48 could potentially be used individually as adjunct treatments for mild DFIs. However, further studies are needed to confirm these findings and assess the potential toxicity and efficacy of PUREG4OEI48 in more complex models.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1937815
dc.identifier.doi10.3390/antibiotics14050444
dc.identifier.issn2079-6382
dc.identifier.otherPURE: 123205486
dc.identifier.otherPURE UUID: defc456e-0ac1-4bb1-90fd-ee001010c57d
dc.identifier.otherScopus: 105006773625
dc.identifier.otherWOS: 001495874300001
dc.identifier.otherPubMed: 40426511
dc.identifier.otherPubMedCentral: PMC12108245
dc.identifier.otherORCID: /0000-0002-4096-2410/work/192930708
dc.identifier.urihttp://hdl.handle.net/10362/188731
dc.identifier.urlhttps://www.scopus.com/pages/publications/105006773625
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAmlodipine
dc.subjectcore–shell polycationic polyurea pharmadendrimer
dc.subjectdiabetic foot infection
dc.subjectGalleria mellonella
dc.subjectNisin Z
dc.subjectPseudomonas aeruginosa
dc.subjectStaphylococcus aureus
dc.subjectMicrobiology
dc.subjectBiochemistry
dc.subjectPharmacology, Toxicology and Pharmaceutics(all)
dc.subjectMicrobiology (medical)
dc.subjectInfectious Diseases
dc.subjectPharmacology (medical)
dc.subjectSDG 3 - Good Health and Well-being
dc.titleIn Vivo Antimicrobial Activity of Nisin Z Against S. aureus and Polyurea Pharmadendrimer PUREG4OEI48 Against P. aeruginosa from Diabetic Foot Infectionsen
dc.typejournal article
degois.publication.issue5
degois.publication.titleAntibiotics
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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