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Antioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systems

2021-09-01Artigo científico Acesso aberto
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dc.contributor.authorGuerreiro, Bruno M.
dc.contributor.authorSilva, Jorge Carvalho
dc.contributor.authorLima, João Carlos
dc.contributor.authorReis, Maria A. M.
dc.contributor.authorFreitas, Filomena
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionLAQV@REQUIMTE
dc.contributor.institutionDF – Departamento de Física
dc.contributor.institutionCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-10-22T03:43:35Z
dc.date.available2021-10-22T03:43:35Z
dc.date.issued2021-09-01
dc.descriptionUIDP/04378/2020 UIDB/04378/2020 SFRH/BD/144258/2019
dc.description.abstractReactive oxygen species (ROS) are dangerous sources of macromolecular damage. While most derive from mitochondrial oxidative phosphorylation, their production can be triggered by ex-ogenous stresses, surpassing the extinction capacity of intrinsic antioxidant defense systems of cells. Here, we report the antioxidant activity of FucoPol, a fucose-rich polyanionic polysaccharide produced by Enterobacter A47, containing ca. 17 wt% of negatively charged residues in its struc-ture. Ferric reducing antioxidant power (FRAP) assays coupled to Hill binding kinetics fitting have shown FucoPol can neutralize ferricyanide and Fe3+-TPTZ species at an EC50 of 896 and 602 µg/mL, respectively, with positive binding cooperativity (2.52 ≤ H ≤ 4.85). This reducing power is greater than most polysaccharides reported. Moreover, an optimal 0.25% w/v FucoPol concentration shown previously to be cryo-and photoprotective was also demonstrated to protect Vero cells against H2O2-induced acute exposure not only by attenuating metabolic viability decay, but also by accentuating post-stress proliferation capacity, whilst preserving cell morphology. These results on antioxidant activity provide evidence for the biopolymer’s ability to prevent positive feedback cascades of the radical-producing Fenton reaction. Ultimately, FucoPol provides a biotechnological alternative for implementation in cryopreservation, food supplementation, and photoprotective sunscreen formula design, as all fields benefit from an antioxidant functionality.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1474089
dc.identifier.doi10.3390/polym13183020
dc.identifier.otherPURE: 34359738
dc.identifier.otherPURE UUID: 78cbf3e1-df98-4b91-832e-98d4bcec8d87
dc.identifier.otherScopus: 85114708034
dc.identifier.otherPubMed: 34577923
dc.identifier.otherPubMedCentral: PMC8470694
dc.identifier.otherWOS: 000699931400001
dc.identifier.otherORCID: /0000-0001-9959-4272/work/101864211
dc.identifier.otherORCID: /0000-0002-9430-4640/work/101864445
dc.identifier.otherORCID: /0000-0003-0528-1967/work/101864523
dc.identifier.urihttp://hdl.handle.net/10362/126497
dc.identifier.urlhttps://www.scopus.com/pages/publications/85114708034
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147218/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147333/PT
dc.subjectAntioxidant
dc.subjectCryopreservation
dc.subjectFood
dc.subjectFRAP
dc.subjectFucoPol
dc.subjectHill
dc.subjectHydrogen peroxide
dc.subjectPolysaccharide
dc.subjectRadiation
dc.subjectStress
dc.titleAntioxidant potential of the bio-based fucose-rich polysaccharide fucopol supports its use in oxidative stress-inducing systemsen
dc.typejournal article
degois.publication.issue18
degois.publication.titlePolymers
degois.publication.volume13
dspace.entity.typePublication
oaire.awardNumber147218
oaire.awardNumber147333
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/147218/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/147333/PT
oaire.fundingStream5876
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication3875880c-0b37-45f2-a4ac-cb5f59861c2d
relation.isProjectOfPublication4feabffc-4782-4bcd-8f7d-aaac8c3f2325
relation.isProjectOfPublication.latestForDiscovery4feabffc-4782-4bcd-8f7d-aaac8c3f2325

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