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Taxonomic Diversity and Metabolic and Pharmacological Profiles of Marine-Derived Actinomycetes from the Lisbon and Setúbal Coast, Portugal

2026-02-03Artigo científico Acesso aberto
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dc.contributor.authorCoelho, Miguel P.
dc.contributor.authorSuárez-Moo, Pablo
dc.contributor.authorRocha, Mariana
dc.contributor.authorMatos, Artur O.G.
dc.contributor.authorMarques, Vanda
dc.contributor.authorMargarida, Sara
dc.contributor.authorMil-Homens, Mário
dc.contributor.authorPrieto-Davó, Alejandra
dc.contributor.authorRodrigues, Cecília M.P.
dc.contributor.authorBauermeister, Anelize
dc.contributor.authorSobral, Rita G.
dc.contributor.authorGaudêncio, Susana P.
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2026-07-13T14:11:01Z
dc.date.available2026-07-13T14:11:01Z
dc.date.issued2026-02-03
dc.description.abstractThis study explores the taxonomic diversity, metabolic profile, and bioactivity of marine-derived actinomycetes isolated from sediments collected off the coast of Lisbon and Setúbal Peninsula, Portugal. The combined use of two sediment pre-treatments (heat shock and dry overnight) and four growth media with varying nutrient concentrations revealed that formulations 10% A1 and SWA were most effective for recovering diverse actinomycetes, including rare Actinomadura, resulting in a total of 142 cultivable strains closely related to 47 phylogenetic distinct species dominated by Streptomyces and Micromonospora. Antimicrobial screening against methicillin-resistant Staphylococcus aureus (MRSA, COL) and Escherichia coli (K12) identified 22 bioactive strains, with strain PTS-083 exhibiting the strongest activity against MRSA (MIC = 1.95 µg/mL) and a 98.30% 16S rRNA gene identity to S. chumphonensis, highlighting it as a strong candidate for further metabolite and genomic studies. Cytotoxicity assays against HCT-116 human colorectal adenocarcinoma cells revealed eight bioactive strains with potent anticancer activity for extracts from strains related to S. sundarbansensis, S. violaceorubidus, and S. aculeolatus (IC50 < 0.005–5.08 µg/mL). Untargeted LC-MS/MS metabolomic analysis uncovered a wide array of secondary metabolites, including macrolides, siderophores, fatty acids, and cyclic peptides. Comparative analyses with other Portuguese coastal studies revealed both shared and distinctive metabolomic profiles, emphasizing the importance of exhaustive sampling, even at nearby locations, since localized environmental conditions can influence metabolic diversity and are crucial for uncovering unique metabolites with potential biotechnological value. These findings highlight Portugal’s coastal sediments as a rich and underexplored source of novel actinomycetes and bioactive compounds with promising pharmaceutical applications.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent22
dc.format.extent7740511
dc.identifier.doi10.3390/md24020068
dc.identifier.issn1660-3397
dc.identifier.otherPURE: 153299294
dc.identifier.otherPURE UUID: 5aa0c1bc-eedb-4c99-a731-6ef7a0d22a39
dc.identifier.otherORCID: /0000-0002-5510-1170/work/205037143
dc.identifier.otherScopus: 105031269998
dc.identifier.otherPubMed: 41745471
dc.identifier.otherWOS: 001701424400001
dc.identifier.otherORCID: /0000-0003-4533-7531/work/220580655
dc.identifier.urihttp://hdl.handle.net/10362/204455
dc.identifier.urlhttps://www.mdpi.com/1660-3397/24/2/68
dc.identifier.urlhttps://www.scopus.com/pages/publications/105031269998
dc.language.isoeng
dc.peerreviewedyes
dc.subjectactinobacteria
dc.subjectantimicrobial and anticancer activity
dc.subjectblue biotechnology
dc.subjectmarine natural products
dc.subjectmetabolomics
dc.subjectmolecular networking
dc.subjectPharmaceutical Science
dc.subjectDrug Discovery
dc.subjectPharmacology, Toxicology and Pharmaceutics (miscellaneous)
dc.subjectSDG 14 - Life Below Water
dc.titleTaxonomic Diversity and Metabolic and Pharmacological Profiles of Marine-Derived Actinomycetes from the Lisbon and Setúbal Coast, Portugalen
dc.typejournal article
degois.publication.issue2
degois.publication.titleMarine Drugs
degois.publication.volume24
dspace.entity.typePublication
rcaap.rightsopenAccess

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