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Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB

2021-06-08Artigo científico Acesso aberto
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dc.contributor.authorEscrevente, Cristina
dc.contributor.authorBento-Lopes, Liliana
dc.contributor.authorRamalho, José S
dc.contributor.authorBarral, Duarte C
dc.contributor.authorC. Barral, Duarte
dc.contributor.institutioniNOVA4Health - pólo NMS
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.pblCompany of Biologists Ltd
dc.date.accessioned2021-07-05T22:18:09Z
dc.date.available2021-07-05T22:18:09Z
dc.date.issued2021-06-08
dc.descriptionFunding: This study was supported by Fundaçã o para a Ciência e Tecnologia (FCT): C.E. was supported by a post-doctoral fellowship (SFRH/BPD/78491/2011), L.B.-L. by a PhD fellowship (SFRH/BD/131938/2017) and D.C.B. by the FCT Investigator Program (IF/00501/2014/CP1252/CT0001). This work was developed with the support from the research infrastructure PPBI-POCI-01-0145-FEDER-022122, co-financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund). This article was supported by the LYSOCIL project. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 811087. Deposited in PMC for immediate release.
dc.description.abstractLysosomes are dynamic organelles, capable of undergoing exocytosis. This process is crucial for several cellular functions, namely plasma membrane repair. Nevertheless, the molecular machinery involved in this process is poorly understood. Here, we identify Rab11a and Rab11b as regulators of calcium-induced lysosome exocytosis. Interestingly, Rab11-positive vesicles transiently interact with lysosomes at the cell periphery, indicating that this interaction is required for the last steps of lysosome exocytosis. Additionally, we found that the silencing of the exocyst subunit Sec15, a Rab11 effector, impairs lysosome exocytosis, suggesting that Sec15 acts together with Rab11 in the regulation of lysosome exocytosis. Furthermore, we show that Rab11 binds the guanine nucleotide exchange factor for Rab3a (GRAB) and also Rab3a, which we described previously as a regulator of the positioning and exocytosis of lysosomes. Thus, our study identifies new players required for lysosome exocytosis and suggest the existence of a Rab11-Rab3a cascade involved in this process.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent4133907
dc.identifier.doi10.1242/jcs.246694
dc.identifier.issn0021-9533
dc.identifier.otherPURE: 31942675
dc.identifier.otherPURE UUID: 5fa9a01f-7f0e-48cb-ba13-122576ea7752
dc.identifier.otherPubMed: 34002205
dc.identifier.otherScopus: 85107887284
dc.identifier.otherWOS: 000663030200001
dc.identifier.otherPubMed: 34100549
dc.identifier.otherORCID: /0000-0001-8867-2407/work/123561665
dc.identifier.urihttp://hdl.handle.net/10362/120539
dc.language.isoeng
dc.peerreviewedyes
dc.subjectExocytosis
dc.subjectGRAB
dc.subjectRAB3IL1
dc.subjectLysosome
dc.subjectRab11
dc.subjectRab3a
dc.subjectSec15
dc.titleRab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRABen
dc.typejournal article
degois.publication.issue11
degois.publication.titleJournal of Cell Science
degois.publication.volume134
dspace.entity.typePublication
person.familyNameBarral
person.givenNameDuarte
person.identifier.ciencia-id8810-DEBC-025E
person.identifier.orcid0000-0001-8867-2407
person.identifier.ridE-7548-2013
person.identifier.scopus-author-id6602513159
rcaap.rightsopenAccess
relation.isAuthorOfPublicatione8205a0a-7fb2-450a-9917-417ec75d88b3
relation.isAuthorOfPublication.latestForDiscoverye8205a0a-7fb2-450a-9917-417ec75d88b3

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