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A One-Pot, Whole-Cell Biocatalysis Approach for Vanillin Production using Lignin Oil

2023-11-21Artigo científico Acesso aberto
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dc.contributor.authorMarić, Ivana
dc.contributor.authorGuo, Yiming
dc.contributor.authorFürst, Maximilian J.L.J.
dc.contributor.authorVan Aelst, Korneel
dc.contributor.authorVan den Bosch, Sander
dc.contributor.authorDe Simone, Mario
dc.contributor.authorMartins, Lígia O.
dc.contributor.authorSels, Bert F.
dc.contributor.authorFraaije, Marco W.
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblJohn Wiley & Sons, Ltd.
dc.date.accessioned2024-04-02T23:52:07Z
dc.date.available2024-04-02T23:52:07Z
dc.date.issued2023-11-21
dc.descriptionFunding Information: This project has received funding from the Bio‐based Industries Joint Undertaking (JU) under the European Union's Horizon 2020 research and innovation programme under grant agreement No 837890 (Smartbox project). The JU receives support from the European Union's Horizon 2020 research and innovation programme and the Bio‐based Industries Consortium. The support provided by China Scholarship Council (CSC No. 2018201808140173) during a study of Yiming Guo at University of Groningen is acknowledged. Lignin oil was kindly provided by Dr. Korneel Van Aelst & Dr. Sander Van den Bosch (KU Leuven, Belgium). Publisher Copyright: © 2023 The Authors. Advanced Synthesis & Catalysis published by Wiley-VCH GmbH.
dc.description.abstractVanillin is a popular and versatile flavor compound, almost entirely produced from petroleum-derived phenol by a multi-step chemical synthesis. The process is hazardous to the environment and unsustainable for its fossil oil usage. Therefore, developing environmentally friendly, efficient, and sustainable routes to biobased vanillin is essential. Here, we report on vanillin production from 4-n-propylguaiacol (4PG), one of the main components in lignin oil obtained through reductive catalytic fractionation (RCF) of soft wood, by employing recombinant Escherichia coli cells. Conversion is based on the expression of two engineered oxidative enzymes: a 4-n-propylguaiacol oxidase and an isoeugenol dioxygenase. A high yield of vanillin, 66% from 4PG in RCF lignin oil was achieved through rounds of optimisation of the whole-cell conversion process. This high-performance strategy was readily scaled up to produce vanillin at an unprecedented 18% and 3% yield based on lignin oil and spruce wood respectively. The whole-cell bioconversion process shows good tolerance even at high loadings of starting material, showcasing the robustness and applicability of the employed biocatalysts. This work paves the way for further development towards the efficient production of high-titer biobased vanillin using depolymerised lignin as the feedstock.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent9
dc.format.extent5579141
dc.identifier.doi10.1002/adsc.202300868
dc.identifier.issn1615-4150
dc.identifier.otherPURE: 83715158
dc.identifier.otherPURE UUID: d040a155-4fe3-46e4-9d26-e4285f2d7fac
dc.identifier.otherScopus: 85174718379
dc.identifier.urihttp://hdl.handle.net/10362/165741
dc.identifier.urlhttps://www.scopus.com/pages/publications/85174718379
dc.language.isoeng
dc.peerreviewedyes
dc.subjectbiocatalysis
dc.subjectdioxygenase
dc.subjectlignin
dc.subjectoxidase
dc.subjectvanillin
dc.subjectCatalysis
dc.subjectOrganic Chemistry
dc.titleA One-Pot, Whole-Cell Biocatalysis Approach for Vanillin Production using Lignin Oilen
dc.typejournal article
degois.publication.firstPage3987
degois.publication.issue22
degois.publication.lastPage3995
degois.publication.titleAdvanced Synthesis and Catalysis
degois.publication.volume365
dspace.entity.typePublication
rcaap.rightsopenAccess

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