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Investigation of the protective suitability of a dental fluorinated varnish by means of X Ray fluorescence and Raman spectroscopy

dc.contributor.authorOtel, Iulian
dc.contributor.authorDias, Kateline Marisa
dc.contributor.authorPereira, Ruben
dc.contributor.authorFonseca, Micaela
dc.contributor.authorJesus, A. P.
dc.contributor.authorMata, António
dc.contributor.authorVassilenko, Valentina
dc.contributor.authorSilveira, João Miguel
dc.contributor.authorPessanha, Sofia
dc.contributor.institutionLIBPhys-UNL
dc.contributor.pblElsevier Science B.V., Amsterdam.
dc.date.accessioned2022-03-22T23:27:07Z
dc.date.available2023-02-02T01:32:01Z
dc.date.embargoedUntil2023-02-01
dc.date.issued2022-05
dc.descriptionThis work has been financially supported by Fundação para a Ciência e a Tecnologia through the LIBPhys funding UID/FIS/04559/2020 Publisher Copyright: © 2022
dc.description.abstractBackground and aim: Evaluating the protective effect in human enamel of a fluorinated varnish after enduring a citric acid erosive challenge. Methods: An in vitro model was developed considering the intraoral environment, human saliva and acid erosive procedures. The evaluation of the enamel specimens was undertaken through the direct analysis of enamel by means of Raman spectroscopy and Energy Dispersive X Ray Fluorescence (EDXRF). Ten tooth specimens per group were analysed during three stages: 1- before treatment; 2- After varnish (treatment group) or toothpaste (control) application; 3- After citric acid cycle. Additionally, Particle Induced Gamma Ray emission (PIGE) was used to gauge the fluorine uptake by enamel after the application of the varnish (stage 2). Results were presented as mean and standard deviation with ANOVA and Tukey post hoc performed considering a significance level of 0.05. Results: A significant (p < 0.05) higher Ca levels were detected in treatment group at stage 2 (37.4 ± 0.4 w/w%) and 3 (37.1 ± 0.1) when compared to the control group. After varnish application in treatment group, depolarization ratios were significant lower (p < 0.05) and anisotropy were significant higher (p < 0.05), however no differences were detected in FWHM. Conclusions: The use of a fluorinated dental varnish suggests a protective effect for human enamel against dental erosion demineralization process which was detectable in an in vitro model.en
dc.description.versionauthorsversion
dc.description.versionpublished
dc.format.extent953765
dc.identifier.doi10.1016/j.jtemb.2022.126938
dc.identifier.issn0946-672X
dc.identifier.otherPURE: 36711741
dc.identifier.otherPURE UUID: b43c0da8-58ff-4276-a5f2-0d8c6ce1bdc0
dc.identifier.otherScopus: 85123758053
dc.identifier.otherPubMed: 35114575
dc.identifier.otherORCID: /0000-0002-6039-2494/work/109668779
dc.identifier.otherORCID: /0000-0001-7946-4825/work/109669446
dc.identifier.urihttp://hdl.handle.net/10362/135040
dc.identifier.urlhttps://www.scopus.com/pages/publications/85123758053
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: info:eu-repo/grantAgreement/FCT/OE/PD%2FBDE%2F143107%2F2018/PT
dc.subjectAcidic cycle
dc.subjectCalcium
dc.subjectEnamel
dc.subjectFluorine
dc.subjectPhosphate
dc.subjectBiochemistry
dc.subjectMolecular Medicine
dc.subjectInorganic Chemistry
dc.titleInvestigation of the protective suitability of a dental fluorinated varnish by means of X Ray fluorescence and Raman spectroscopyen
dc.typejournal article
degois.publication.titleJournal Of Trace Elements In Medicine And Biology
degois.publication.volume71
dspace.entity.typePublication
rcaap.rightsopenAccess

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