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Challenges of the Application of In Vitro Digestion for Nanomaterials Safety Assessment

2024-06Artigo científico Acesso aberto
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dc.contributor.authorVital, Nádia
dc.contributor.authorGramacho, Ana Catarina
dc.contributor.authorSilva, Mafalda
dc.contributor.authorCardoso, Maria
dc.contributor.authorAlvito, Paula
dc.contributor.authorKranendonk, Michel
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionCentre for Toxicogenomics and Human Health (ToxOmics)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2024-06-20T22:27:17Z
dc.date.available2024-06-20T22:27:17Z
dc.date.issued2024-06
dc.descriptionPublisher Copyright: © 2024 by the authors.
dc.description.abstractConsidering the increase in the production and use of nanomaterials (NM) in food/feed and food contact materials, novel strategies for efficient and sustainable hazard characterization, especially in the early stages of NM development, have been proposed. Some of these strategies encompass the utilization of in vitro simulated digestion prior to cytotoxic and genotoxic assessment. This entails exposing NM to fluids that replicate the three successive phases of digestion: oral, gastric, and intestinal. Subsequently, the resulting digestion products are added to models of intestinal cells to conduct toxicological assays, analyzing multiple endpoints. Nonetheless, exposure of intestinal cells to the digested products may induce cytotoxicity effects, thereby posing a challenge to this strategy. The aim of this work was to describe the challenges encountered with the in vitro digestion INFOGEST 2.0 protocol when using the digestion product in toxicological studies of NM, and the adjustments implemented to enable its use in subsequent in vitro biological assays with intestinal cell models. The adaptation of the digestion fluids, in particular the reduction of the final bile concentration, resulted in a reduced toxic impact of digestion products.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1282034
dc.identifier.doi10.3390/foods13111690
dc.identifier.issn2304-8158
dc.identifier.otherPURE: 93683273
dc.identifier.otherPURE UUID: 0bf6b0a6-ba4d-48eb-9bed-577774328b93
dc.identifier.otherScopus: 85195849594
dc.identifier.otherPubMed: 38890918
dc.identifier.otherWOS: 001245350700001
dc.identifier.urihttp://hdl.handle.net/10362/168860
dc.identifier.urlhttps://www.scopus.com/pages/publications/85195849594
dc.language.isoeng
dc.peerreviewedyes
dc.subjectCaco-2
dc.subjectdigestion product
dc.subjectin vitro simulated digestion
dc.subjectINFOGEST
dc.subjectnanomaterials
dc.subjectFood Science
dc.subjectMicrobiology
dc.subjectHealth(social science)
dc.subjectHealth Professions (miscellaneous)
dc.subjectPlant Science
dc.titleChallenges of the Application of In Vitro Digestion for Nanomaterials Safety Assessmenten
dc.typejournal article
degois.publication.issue11
degois.publication.titleFoods
degois.publication.volume13
dspace.entity.typePublication
rcaap.rightsopenAccess

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