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Dynamics in the profile of biopolymers produced by mixed microbial cultures from ethanol-rich feedstocks

dc.contributor.authorRangel, Catarina
dc.contributor.authorLourenço, Nídia D.
dc.contributor.authorReis, Maria A. M.
dc.contributor.authorCarvalho, Gilda
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblElsevier BV
dc.date.accessioned2024-09-23T22:22:49Z
dc.date.available2024-09-23T22:22:49Z
dc.date.issued2024-06
dc.descriptionPublisher Copyright: © 2024 The Authors
dc.description.abstractThe use of agro-industrial wastes as substrates for polyhydroxyalkanoate (PHA) production by mixed microbial cultures is an alternative to lower the price of these biopolymers. However, when submitting these wastes to pre-fermentation, lactate and ethanol can be produced in addition to volatile fatty acids (VFAs). In this study, the impact of ethanol on PHA storage and in the carbon flux of the process were assessed. The co-production of extracellular polymeric substances (EPS) and its impact on PHA production was investigated. PHA production yield showed a decrease from 0.92 ± 0.004–0.63 ± 0.002 Cmol-PHA.Cmol-S−1 with the increase of ethanol content from 5 to 20 Cmmol.L−1. EPS production was directly proportional to the ethanol's fed, reaching a maximum EPS total content of 6.70 Cmmol for the maximum ethanol supplied. EPS acted as a protection strategy, which benefited PHA specific storage rate for the highest ethanol content tested. These results indicate that ethanol-rich feedstocks can be valorised through the simultaneous production of two value-added biopolymers.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent9
dc.format.extent2596779
dc.identifier.doi10.1016/j.jece.2024.112609
dc.identifier.issn2213-3437
dc.identifier.otherPURE: 99834409
dc.identifier.otherPURE UUID: 0ae91337-8c0d-41b5-b32d-2ac377dca026
dc.identifier.otherScopus: 85189745024
dc.identifier.otherWOS: 001227323600001
dc.identifier.urihttp://hdl.handle.net/10362/172278
dc.identifier.urlhttps://www.scopus.com/pages/publications/85189745024
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: info:eu-repo/grantAgreement/EC/H2020/745737/EU
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationEnhanced polyhydroxyalkanoates productivity from industrial wastes: knowledge-based process design and online control
dc.relationinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F133033%2F2017/PT
dc.subjectEthanol
dc.subjectExtracellular polymeric substances (EPS)
dc.subjectMixed microbial cultures (MMC)
dc.subjectPolyhydroxyalkanoates (PHA)
dc.subjectChemical Engineering (miscellaneous)
dc.subjectWaste Management and Disposal
dc.subjectPollution
dc.subjectProcess Chemistry and Technology
dc.titleDynamics in the profile of biopolymers produced by mixed microbial cultures from ethanol-rich feedstocksen
dc.typejournal article
degois.publication.issue3
degois.publication.titleJournal of Environmental Chemical Engineering
degois.publication.volume12
dspace.entity.typePublication
oaire.awardNumberUIDP/04378/2020
oaire.awardNumberUIDB/04378/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardNumberSFRH/BD/133033/2017
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleEnhanced polyhydroxyalkanoates productivity from industrial wastes: knowledge-based process design and online control
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F133033%2F2017/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication38373452-5c9c-4724-8def-f03314ecce0e
relation.isProjectOfPublicatione07cf232-4705-4b5b-b2c4-af8f25311076
relation.isProjectOfPublication834c369e-6900-4105-aa78-6ba0e52f96ca
relation.isProjectOfPublication1059c949-3054-4fcb-b4bb-c7bb349aa693
relation.isProjectOfPublication.latestForDiscovery834c369e-6900-4105-aa78-6ba0e52f96ca

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