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Magnetic Precipitation: A New Platform for Protein Purification

2020-09-01Artigo científico Acesso aberto
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dc.contributor.authorDos Santos, Raquel
dc.contributor.authorIria, Inês
dc.contributor.authorManuel, Ana M.
dc.contributor.authorLeandro, Ana P.
dc.contributor.authorMadeira, Catarina A. C.
dc.contributor.authorGoncalves, João
dc.contributor.authorCarvalho, Ana Luísa
dc.contributor.authorRoque, Ana Cecília
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblWiley
dc.date.accessioned2022-07-13T22:19:57Z
dc.date.available2022-07-13T22:19:57Z
dc.date.issued2020-09-01
dc.descriptionPD/BD/105753/2014 UIDB/ 04378/2020 POCI-01-0145-FEDER-007728 PTDC/BII-BIO/28878/2017
dc.description.abstractOne of the trends in downstream processing comprises the use of “anything-but-chromatography” methods to overcome the current downfalls of standard packed-bed chromatography. Precipitation and magnetic separation are two techniques already proven to accomplish protein purification from complex media, yet never used in synergy. With the aim to capture antibodies directly from crude extracts, a new approach combining precipitation and magnetic separation is developed and named as affinity magnetic precipitation. A precipitation screening, based on the Hofmeister series, and a commercial precipitation kit are tested with affinity magnetic particles to assess the best condition for antibody capture from human serum plasma and clarified cell supernatant. The best conditions are obtained when using PEG3350 as precipitant at 4 °C for 1 h, reaching 80% purity and 50% recovery of polyclonal antibodies from plasma, and 99% purity with 97% recovery yield of anti-TNFα mAb from cell supernatants. These results show that the synergetic use of precipitation and magnetic separation can represent an alternative for the efficient capture of antibodies.en
dc.description.versionauthorsversion
dc.description.versionpublished
dc.format.extent1930623
dc.identifier.doi10.1002/biot.202000151
dc.identifier.issn1860-6768
dc.identifier.otherPURE: 18808618
dc.identifier.otherPURE UUID: 7aa5e1f7-f218-482b-83c7-fd56698b6686
dc.identifier.otherPubMed: 32578939
dc.identifier.otherScopus: 85087656742
dc.identifier.otherWOS: 000546027700001
dc.identifier.otherORCID: /0000-0002-3824-0240/work/93243402
dc.identifier.urihttp://hdl.handle.net/10362/141851
dc.identifier.urlhttps://www.scopus.com/pages/publications/85087656742
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/125363/PT info:eu-repo/grantAgreement/EC/FP7/283570/EU
dc.subjectaffinity magnetic precipitation
dc.subjectantibody purification
dc.subjectdownstream
dc.subjectmagnetic nanoparticles
dc.subjectmethod development
dc.subjectApplied Microbiology and Biotechnology
dc.subjectMolecular Medicine
dc.titleMagnetic Precipitation: A New Platform for Protein Purificationen
dc.typejournal article
degois.publication.issue9
degois.publication.titleBiotechnology Journal
degois.publication.volume15
dspace.entity.typePublication
oaire.awardNumberRECI/BBB-BEP/0124/2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/RECI%2FBBB-BEP%2F0124%2F2012/PT info:eu-repo/grantAgreement/EC/FP7/283570/EU
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication8a709e7d-17a1-40da-8d44-f461e28eeecd
relation.isProjectOfPublication.latestForDiscovery8a709e7d-17a1-40da-8d44-f461e28eeecd

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