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Multiple pathways to homothallism in closely related yeast lineages in the basidiomycota

2021-01-01Artigo científico Acesso aberto
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dc.contributor.authorCabrita, Alexandra
dc.contributor.authorDavid-Palma, Márcia
dc.contributor.authorBrito, Patrícia H.
dc.contributor.authorHeitman, Joseph
dc.contributor.authorCoelho, Marco A.
dc.contributor.authorGonçalves, Paula
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblAmerican Society for Microbiology
dc.date.accessioned2021-10-13T04:36:22Z
dc.date.available2021-10-13T04:36:22Z
dc.date.issued2021-01-01
dc.descriptionSFRH/BPD/79198/2011 01/SAICT/2016 grant 022153 NIH/NIAID R37 award AI39115-23 R01 award AI50113-16.
dc.description.abstractSexual reproduction in fungi relies on proteins with well-known functions encoded by the mating type (MAT) loci. In the Basidiomycota, MAT loci are often bipar-tite, with the P/R locus encoding pheromone precursors and pheromone receptors and the HD locus encoding heterodimerizing homeodomain transcription factors (Hd1/Hd2). The interplay between different alleles of these genes within a single species usually gen-erates at least two compatible mating types. However, a minority of species are homo-thallic, reproducing sexually without an obligate need for a compatible partner. Here, we examine the organization and function of the MAT loci of Cystofilobasidium capitatum, a species in the order Cystofilobasidiales, which is unusually rich in homothallic species. We determined MAT gene content and organization in C. capitatum and found that it resembles a mating type of the closely related heterothallic species Cystofilobasidium feri-gula. To explain the homothallic sexual reproduction observed in C. capitatum, we exam-ined HD protein interactions in the two Cystofilobasidium species and determined C. capi-tatum MAT gene expression both in a natural setting and upon heterologous expression in Phaffia rhodozyma, a homothallic species belonging to a clade sister to that of Cystofilobasidium. We conclude that the molecular basis for homothallism in C. capitatum appears to be distinct from that previously established for P. rhodozyma. Unlike in the latter species, homothallism in C. capitatum may involve constitutive activation or dis-pensability of the pheromone receptor and the functional replacement of the usual Hd1/ Hd2 heterodimer by an Hd2 homodimer. Overall, our results suggest that homothallism evolved multiple times within the Cystofilobasidiales.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent15
dc.format.extent2114379
dc.identifier.doi10.1128/mBio.03130-20
dc.identifier.issn2161-2129
dc.identifier.otherPURE: 34234677
dc.identifier.otherPURE UUID: 528be11b-3845-43c6-81fc-e9f458324b0f
dc.identifier.otherScopus: 85100988339
dc.identifier.otherPubMed: 33593979
dc.identifier.otherWOS: 000627333700006
dc.identifier.otherORCID: /0000-0002-2457-7520/work/151431812
dc.identifier.urihttp://hdl.handle.net/10362/126025
dc.identifier.urlhttps://www.scopus.com/pages/publications/85100988339
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04378%2F2019/PT
dc.relationApplied Molecular Biosciences Unit
dc.relationELUCIDATING THE MATING SYSTEM OF PHAFFIA RHODOZYMA,N ASTAXANTHIN PRODUCING YEAST WITH BIOTECHNOLOGICAL POTENTIAL
dc.subjectCystofilobasidiales
dc.subjectCystofilobasidium
dc.subjectEvolution of sexual reproduction
dc.subjectHomothallism
dc.subjectMAT loci
dc.subjectPhaffia
dc.subjectSexual reproduction in basidiomycetes
dc.subjectMicrobiology
dc.subjectVirology
dc.titleMultiple pathways to homothallism in closely related yeast lineages in the basidiomycotaen
dc.typejournal article
degois.publication.firstPage1
degois.publication.issue1
degois.publication.lastPage15
degois.publication.titlemBio
degois.publication.volume12
dspace.entity.typePublication
oaire.awardNumberUID/Multi/04378/2019
oaire.awardNumberPTDC/BIA-GEN/112799/2009
oaire.awardNumberSFRH/BD/81895/2011
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleELUCIDATING THE MATING SYSTEM OF PHAFFIA RHODOZYMA,N ASTAXANTHIN PRODUCING YEAST WITH BIOTECHNOLOGICAL POTENTIAL
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04378%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-GEN%2F112799%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F81895%2F2011/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication7fcd6a91-6464-4b87-ad23-8ce45266ae17
relation.isProjectOfPublicationf8023202-2db2-4c9b-bef1-906e8e3ba234
relation.isProjectOfPublication2971f108-84a7-4b60-8d05-e235014bcbed
relation.isProjectOfPublication.latestForDiscovery2971f108-84a7-4b60-8d05-e235014bcbed

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