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Redox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774

1996-09-30Artigo científico Acesso aberto
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dc.contributor.authorCosta, Cristina
dc.contributor.authorMoura, José J. G.
dc.contributor.authorMoura, Isabel
dc.contributor.authorWang, Yaning
dc.contributor.authorHuynh, Boi Hanh
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
dc.contributor.pblASBMB - American Society for Biochemistry and Molecular Biology
dc.date.accessioned2019-09-09T22:37:06Z
dc.date.available2019-09-09T22:37:06Z
dc.date.issued1996-09-30
dc.descriptionNIGMS NIH HHS (GM 39803) NIGMS NIH HHS (GM 47295)
dc.description.abstractThe dissimilatory nitrite reductase from Desulfovibrio desulfuricans ATCC 27774 catalyzes the reduction of nitrite to ammonia. Previous spectroscopic investigation revealed that it is a hexaheme cytochrome containing one high spin ferric heme and five low spin ferric hemes in the oxidized enzyme. The current study uses the high resolution of Mossbauer spectroscopy to obtain redox properties of the six heme groups. Correlating the Mossbauer findings with the EPR data reveals the pair-wise spin-spin coupling among four of the heme groups. The other two hemes are found to be magnetically isolated. Reduction with dithionite and reaction with CO further indicate that only the high spin heme is capable of binding small exogenous ligands. These results confirm our previous finding that Desulfovibrio desulfuricans nitrite reductase contains six heme groups and that the high spin ferric heme is the substrate and inhibitor binding site.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent6
dc.format.extent228798
dc.identifier.doi10.1074/jbc.271.38.23191
dc.identifier.issn0021-9258
dc.identifier.otherPURE: 14620899
dc.identifier.otherPURE UUID: 9a35afe4-bd50-4dc5-836f-af6fcd8e6b6f
dc.identifier.otherScopus: 0029841950
dc.identifier.otherPubMed: 8798514
dc.identifier.otherWOS: A1996VH76800040
dc.identifier.otherORCID: /0000-0002-8611-9023/work/62320637
dc.identifier.otherORCID: /0000-0002-4726-2388/work/68772360
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=0029841950&partnerID=8YFLogxK
dc.identifier.urlhttps://www.scopus.com/pages/publications/0029841950
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectCell Biology
dc.titleRedox properties of cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774en
dc.typejournal article
degois.publication.firstPage23191
degois.publication.issue38
degois.publication.lastPage23196
degois.publication.titleJournal of Biological Chemistry
degois.publication.volume271
dspace.entity.typePublication
rcaap.rightsopenAccess

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