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Bioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbon

2020Artigo científico Acesso aberto
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dc.contributor.authorViegas, Carla
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorLourenço, Raquel
dc.contributor.authorDias, Marta
dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Anita Quintal
dc.contributor.authorKosicki, Robert
dc.contributor.authorSoszczyńska, Ewelina
dc.contributor.authorViegas, Susana
dc.contributor.institutionComprehensive Health Research Centre (CHRC) - Pólo ENSP
dc.contributor.institutionCentro de Investigação em Saúde Pública (CISP/PHRC)
dc.contributor.institutionEscola Nacional de Saúde Pública (ENSP)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2020-04-06T22:34:00Z
dc.date.available2020-04-06T22:34:00Z
dc.date.issued2020
dc.description.abstractThe assessment and control of microbial contamination in health care facilities is presently a mandatory and vital part of strategies to prevent and control hospital-acquired infections. This study aims to assess the bioburden with two passive sampling methods (30 ventilations grids swabs and 16 electrostatic dust collectors (EDCs) at Clinical Pathology Services. The fungal burden was characterized through molecular tools, antifungal resistance, and the mycotoxins and cytotoxicity profile. Total bacteria presented the highest prevalence in both matrixes, whereas Gram-bacteria presented the lowest. Swabs presented a higher prevalence (27.6%) for fungal burden. Chrysonilia sitophila presented the highest prevalence in swabs, whereas for EDCs, C. sitophila and Mucor sp. were the most prevalent. Concerning Aspergillus genera on swabs, section Flavi was the one with the highest prevalence (58.02%), whereas, for EDCs, section Versicolores was the only section observed (100%). Aspergillus section Fumigati was detected in 10 swabs and 7 EDC samples and Aspergillus section Versicolores was detected in one EDC sample. Fungal growth on azole-supplemented media was observed in eight EDC samples. No mycotoxins were detected in any of the samples. A low cytotoxic effect was observed in two sites upon incubation of collected samples with A549 and SK cells and in two other sites upon incubation of collected samples with SK cells only. A medium cytotoxic effect was observed with one EDC sample upon incubation with A549 cells. This study reinforces the need of determination of the azole resistance profile for fungal species and allowed a preliminary risk characterization regarding the cytotoxicity. An intervention including the use of a ultraviolet with wavelength between 200 nm and 280 nm (UVC)-emitting device and an increased maintenance and cleaning of the central heating, ventilation, and air conditioning (HVAC) systems should be ensured to promote the reduction of microbial contamination.en
dc.description.versionpublishersversion
dc.description.versionepub_ahead_of_print
dc.format.extent952992
dc.identifier.doi10.3390/atmos11040351
dc.identifier.issn2073-4433
dc.identifier.otherPURE: 17644375
dc.identifier.otherPURE UUID: 94a58222-0149-4096-96ea-47bb4946a439
dc.identifier.othercrossref: 10.3390/atmos11040351
dc.identifier.otherWOS: 000539492200038
dc.identifier.otherScopus: 85085099416
dc.identifier.urihttp://hdl.handle.net/10362/95791
dc.identifier.urlhttps://www.mdpi.com/2073-4433/11/4/351
dc.language.isoeng
dc.peerreviewedyes
dc.titleBioburden assessment by passive methods on a clinical pathology service in one central hospital from Lisbonen
dc.title.subtitlewhat can it tell us regarding patients and staff exposure?en
dc.typejournal article
degois.publication.issue4
degois.publication.titleAtmosphere
degois.publication.volume11
dspace.entity.typePublication
rcaap.rightsopenAccess

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