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The host endocytic pathway is essential for plasmodium berghei late liver stage development

2012-10-01Artigo científico Acesso aberto
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dc.contributor.authorLopes da Silva, Mafalda
dc.contributor.authorThieleke-Matos, Carolina
dc.contributor.authorCabrita-Santos, Laura
dc.contributor.authorRamalho, José S.
dc.contributor.authorWavre-Shapton, Silène T.
dc.contributor.authorFutter, Clare E.
dc.contributor.authorBarral, Duarte C.
dc.contributor.authorC. Barral, Duarte
dc.contributor.authorSeabra, Miguel C.
dc.contributor.authorC Seabra, Miguel
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.pblBlackwell Publishing Ltd
dc.date.accessioned2024-11-07T22:26:56Z
dc.date.available2024-11-07T22:26:56Z
dc.date.issued2012-10-01
dc.description.abstractThe obligate intracellular liver stage of the Plasmodium parasite represents a bottleneck in the parasite life cycle and remains a promising target for therapeutic intervention. During this stage, parasites undergo dramatic morphological changes and achieve one of the fastest replication rates among eukaryotic species. Nevertheless, relatively little is known about the parasite interactions with the host hepatocyte. Using immunofluorescence, live cell imaging and electron microscopy, we show that Plasmodium berghei parasites are surrounded by vesicles from the host late endocytic pathway. We found that these vesicles are acidic and contain the membrane markers Rab7a, CD63 and LAMP1. When host cell vesicle acidification was disrupted using ammonium chloride or Concanamycin A during the late liver stage of infection, parasite survival was not affected, but schizont size was significantly decreased. Furthermore, when the host cell endocytic pathway was loaded with BSA-gold, gold particles were found within the parasite cytoplasm, showing the transport of material from the host endocytic pathway toward the parasite interior. These observations reveal a novel Plasmodium-host interaction and suggest that vesicles from the host endolysosomal pathway could represent an important source of nutrients exploited by the fast-growing late liver stage parasites.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent13
dc.format.extent1832719
dc.identifier.doi10.1111/j.1600-0854.2012.01398.x
dc.identifier.issn1398-9219
dc.identifier.otherPURE: 5410121
dc.identifier.otherPURE UUID: 623effee-3c50-467f-a578-0c10aa94fb7a
dc.identifier.otherScopus: 84866290594
dc.identifier.otherPubMed: 22780869
dc.identifier.otherWOS: 000308882700005
dc.identifier.otherORCID: /0000-0001-8867-2407/work/123561653
dc.identifier.urihttp://hdl.handle.net/10362/174801
dc.identifier.urlhttps://www.scopus.com/pages/publications/84866290594
dc.language.isoeng
dc.peerreviewedyes
dc.subjectEndocytic pathway
dc.subjectLate endosome
dc.subjectLiver stage infection
dc.subjectLysosome
dc.subjectMalaria
dc.subjectPlasmodium berghei
dc.subjectBiochemistry
dc.subjectCell Biology
dc.subjectStructural Biology
dc.subjectMolecular Biology
dc.subjectGenetics
dc.subjectSDG 3 - Good Health and Well-being
dc.titleThe host endocytic pathway is essential for plasmodium berghei late liver stage developmenten
dc.typejournal article
degois.publication.firstPage1351
degois.publication.issue10
degois.publication.lastPage1363
degois.publication.titleTraffic
degois.publication.volume13
dspace.entity.typePublication
person.familyNameBarral
person.familyNameSeabra
person.givenNameDuarte
person.givenNameMiguel
person.identifier.ciencia-id8810-DEBC-025E
person.identifier.ciencia-id2512-FAEF-E26F
person.identifier.orcid0000-0001-8867-2407
person.identifier.orcid0000-0002-6404-4892
person.identifier.ridE-7548-2013
person.identifier.ridM-3280-2013
person.identifier.scopus-author-id6602513159
person.identifier.scopus-author-id7004369652
rcaap.rightsopenAccess
relation.isAuthorOfPublicatione8205a0a-7fb2-450a-9917-417ec75d88b3
relation.isAuthorOfPublicationd6eeb095-df2d-4fd0-8241-ab826e5ecb1d
relation.isAuthorOfPublication.latestForDiscoverye8205a0a-7fb2-450a-9917-417ec75d88b3

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