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Characterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Mice

2022-10-15Artigo científico Acesso aberto
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dc.contributor.authorCochicho, Daniela
dc.contributor.authorNunes, Alexandra
dc.contributor.authorGomes, João Paulo
dc.contributor.authorMartins, Luís
dc.contributor.authorCunha, Mário
dc.contributor.authorMedeiros-Fonseca, Beatriz
dc.contributor.authorOliveira, Paula
dc.contributor.authorBastos, Margarida M.S.M.
dc.contributor.authorMedeiros, Rui
dc.contributor.authorMendonça, Joana
dc.contributor.authorVieira, Luis
dc.contributor.authorGil da Costa, Rui M.
dc.contributor.authorFelix, Ana
dc.contributor.authorFelix, Ana
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2022-11-04T22:11:26Z
dc.date.available2022-11-04T22:11:26Z
dc.date.issued2022-10-15
dc.descriptionby Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal) through national funds to iNOVA4Health (UIDB/04462/2020 and UIDP/ 04462/2020); from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Pro- Int. J. Mol. Sci. 2022, 23, 12371 8 of 10 gramme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT). This work was also supported by Fundos FEDER through the Programa Operacional Factores de Competitividade—COMPETE and by Fundos Nacionais through the Fundação para a Ciência e a Tecnologia within the scope of the project UID/BIM/00009/2019 (Centre for Toxicogenomics and Human Health-ToxOmics); and from LA/P/0045/2020 (ALiCE), UIDB/00511/2020 and UIDP/00511/2020 (LEPABE), funded by national funds through FCT/MCTES (PIDDAC); 2SMART (NORTE-01-0145- FEDER-000054), supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).
dc.description.abstractThe study of human papillomavirus (HPV)-induced carcinogenesis uses multiple in vivo mouse models, one of which relies on the cytokeratin 14 gene promoter to drive the expression of all HPV early oncogenes. This study aimed to determine the HPV16 variant and sublineage present in the K14HPV16 mouse model. This information can be considered of great importance to further enhance this K14HPV16 model as an essential research tool and optimize its use for basic and translational studies. Our study evaluated HPV DNA from 17 samples isolated from 4 animals, both wild-type (n = 2) and HPV16-transgenic mice (n = 2). Total DNA was extracted from tissues and the detection of HPV16 was performed using a qPCR multiplex. HPV16-positive samples were subsequently whole-genome sequenced by next-generation sequencing techniques. The phylogenetic positioning clearly shows K14HPV16 samples clustering together in the sub-lineage A1 (NC001526.4). A comparative genome analysis of K14HPV16 samples revealed three mutations to the human papillomaviruses type 16 sublineage A1 representative strain. Knowledge of the HPV 16 variant is fundamental, and these findings will allow the rational use of this animal model to explore the role of the A1 sublineage in HPV-driven cancer.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1341752
dc.identifier.doi10.3390/ijms232012371
dc.identifier.issn1422-0067
dc.identifier.otherPURE: 47564481
dc.identifier.otherPURE UUID: e31b11b4-3276-49c2-8622-5acbe03e973a
dc.identifier.otherScopus: 85140812546
dc.identifier.otherPubMed: 36293226
dc.identifier.otherORCID: /0000-0002-2653-2262/work/122157994
dc.identifier.urihttp://hdl.handle.net/10362/145251
dc.identifier.urlhttps://www.scopus.com/pages/publications/85140812546
dc.language.isoeng
dc.peerreviewedyes
dc.subjectcarcinogenesis
dc.subjectHPV16
dc.subjectK14HPV16
dc.subjectlineage
dc.subjectvariant
dc.subjectCatalysis
dc.subjectMolecular Biology
dc.subjectSpectroscopy
dc.subjectComputer Science Applications
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.subjectInorganic Chemistry
dc.subjectSDG 3 - Good Health and Well-being
dc.titleCharacterization of the Human Papillomavirus 16 Oncogenes in K14HPV16 Miceen
dc.title.subtitleSublineage A1 Drives Multi-Organ Carcinogenesisen
dc.typejournal article
degois.publication.issue20
degois.publication.titleInternational Journal of Molecular Sciences
degois.publication.volume23
dspace.entity.typePublication
person.familyNameFelix
person.givenNameAna
person.identifier.ciencia-idA617-A56B-6FC6
person.identifier.orcid0000-0002-2653-2262
person.identifier.scopus-author-id55574221034
rcaap.rightsopenAccess
relation.isAuthorOfPublicatione267d7fd-7e65-4533-9f0a-6fceabcf4b7b
relation.isAuthorOfPublication.latestForDiscoverye267d7fd-7e65-4533-9f0a-6fceabcf4b7b

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