Exploration of Toxins from a Marine Annelid

Rodrigo, Ana P.; Moutinho Cabral, Inês; Alexandre, António; Costa, Pedro M.

http://hdl.handle.net/10362/166671

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Autores

Rodrigo, Ana P.

Moutinho Cabral, Inês

Alexandre, António

Costa, Pedro M.

Resumo(s)

Proteinaceous toxins are peptides or proteins that hold great biotechnological value, evidenced by their ecological role, whether as defense or predation mechanisms. Bioprospecting using bioinformatics and omics may render screening for novel bioactives more expeditious, especially considering the immense diversity of toxin-secreting marine organisms. Eulalia sp. (Annelida: Phyllodocidae), a toxin bearing marine annelid, was recently shown to secrete cysteine-rich protein (Crisp) toxins (hitherto referred to as ‘phyllotoxins’) that can immobilize its prey. By analyzing and validating transcriptomic data, we narrowed the list of isolated full coding sequences of transcripts of the most abundant toxins or accompanying bioactives secreted by the species (the phyllotoxin Crisp, hyaluronidase, serine protease, and peptidases M12A, M13, and M12B). Through homology matching with human proteins, the biotechnological potential of the marine annelid’s toxins and related proteins was tentatively associated with coagulative and anti-inflammatory responses for the peptidases PepM12A, SePr, PepM12B, and PepM13, and with the neurotoxic activity of Crisp, and finally, hyaluronidase was inferred to bear properties of an permeabilizing agent. The in silico analysis succeeded by validation by PCR and Sanger sequencing enabled us to retrieve cDNAs can may be used for the heterologous expression of these toxins.

Descrição

The authors acknowledge the Portuguese Foundation for Science and Technology (FCT) for the grant 2022.00252.CEECIND to A.P.R., the grant 2022.11150.BD to I.M.C., the funding of the WormALL project (PTDC/BTA-BTA/28650/2017), and for supporting the Applied Molecular Biosciences Unit—UCIBIO (UIDP/04378/2020 and UIDB/04378/2020) and the Associate Laboratory Institute for Health and Bioeconomy—i4HB (LA/P/0140/2020). The authors also acknowledge Fundo Azul for co-financing the MARVEN project (FA_05_2017_007). Publisher Copyright: © 2024 by the authors.

Palavras-chave

bioprospecting biotechnology homology matching in silico analysis marine invertebrates toxins Animal Science and Zoology General Veterinary SDG 14 - Life Below Water