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The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stress

2019-12-01Artigo científico Acesso aberto
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dc.contributor.authorSantos, Sandra P.
dc.contributor.authorYang, Yang
dc.contributor.authorRosa, Margarida T.G.
dc.contributor.authorRodrigues, Mafalda A.A.
dc.contributor.authorDe La Tour, Claire Bouthier
dc.contributor.authorSommer, Suzanne
dc.contributor.authorTeixeira, Miguel
dc.contributor.authorCarrondo, Maria A.
dc.contributor.authorCloetens, Peter
dc.contributor.authorAbreu, Isabel A.
dc.contributor.authorRomão, Célia V.
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblNature Publishing Group
dc.date.accessioned2020-01-23T23:40:15Z
dc.date.available2020-01-23T23:40:15Z
dc.date.issued2019-12-01
dc.description.abstractThe bacterium Deinococcus radiodurans is highly resistant to several stress conditions, such as radiation. According to several reports, manganese plays a crucial role in stress protection, and a high Mn/Fe ratio is essential in this process. However, mobilization of manganese and iron, and the role of DNA-binding-proteins-under-starved-conditions during oxidative-stress remained open questions. We used synchrotron-based X-ray fluorescence imaging at nano-resolution to follow element-relocalization upon stress, and its dependency on the presence of Dps proteins, using dps knockout mutants. We show that manganese, calcium, and phosphorus are mobilized from rich-element regions that resemble electron-dense granules towards the cytosol and the cellular membrane, in a Dps-dependent way. Moreover, iron delocalizes from the septum region to the cytoplasm affecting cell division, specifically in the septum formation. These mechanisms are orchestrated by Dps1 and Dps2, which play a crucial role in metal homeostasis, and are associated with the D. radiodurans tolerance against reactive oxygen species.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2999442
dc.identifier.doi10.1038/s41598-019-53140-2
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 15763358
dc.identifier.otherPURE UUID: 67da4eb3-c17c-41ad-a9a9-329ef1b6a618
dc.identifier.otherScopus: 85075342637
dc.identifier.otherPubMed: 31748604
dc.identifier.otherORCID: /0000-0003-4124-6237/work/67534115
dc.identifier.urihttp://hdl.handle.net/10362/91695
dc.identifier.urlhttps://www.scopus.com/pages/publications/85075342637
dc.identifier.urlhttps://www.nature.com/articles/s41598-019-53140-2
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGeneral
dc.titleThe interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stressen
dc.typejournal article
degois.publication.issue1
degois.publication.titleScientific Reports
degois.publication.volume9
dspace.entity.typePublication
rcaap.rightsopenAccess

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