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Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions

2019-04-01Artigo científico Acesso aberto
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dc.contributor.authorFradinho, J. C.
dc.contributor.authorOehmen, A.
dc.contributor.authorReis, M. A. M.
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.pblElsevier
dc.date.accessioned2020-06-03T10:25:41Z
dc.date.available2020-06-03T10:25:41Z
dc.date.issued2019-04-01
dc.descriptionThe authors would also like to acknowledge the Fundacao para a Ciencia e a Tecnologia (Portugal) for funding through SFRH/BPD/101642/2014. co-financed by ERDF under PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728).
dc.description.abstractPolyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30 CmM) and specific light intensities around 20 W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83 ± 0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21 ± 0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30% in <4 h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12%. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20 W/gX are sufficient to drive phototrophic PHA production processes.en
dc.description.versionauthorsversion
dc.description.versionpublished
dc.format.extent8
dc.format.extent953135
dc.identifier.doi10.1016/j.ijbiomac.2018.12.270
dc.identifier.issn0141-8130
dc.identifier.otherPURE: 11474735
dc.identifier.otherPURE UUID: 0e1b312f-04b4-4fee-8b6f-870009208efc
dc.identifier.otherScopus: 85059552216
dc.identifier.otherPubMed: 30610947
dc.identifier.otherWOS: 000460710000121
dc.identifier.urihttp://hdl.handle.net/10362/98795
dc.identifier.urlhttps://www.scopus.com/pages/publications/85059552216
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147258/PT
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/688338/EU
dc.relationInnovative approaches to turn agricultural waste into ecological and economic assets
dc.subjectFermented cheese whey (FCW)
dc.subjectPhototrophic mixed cultures (PMCs)
dc.subjectPolyhydroxyalkanoates (PHA)
dc.subjectPurple phototrophic bacteria (PPB)
dc.subjectVolatile fatty acids (VFAs)
dc.subjectStructural Biology
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.titleImproving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditionsen
dc.typejournal article
degois.publication.firstPage1085
degois.publication.lastPage1092
degois.publication.titleInternational Journal of Biological Macromolecules
degois.publication.volume126
dspace.entity.typePublication
oaire.awardNumberUID/Multi/04378/2013
oaire.awardNumber688338
oaire.awardTitleInnovative approaches to turn agricultural waste into ecological and economic assets
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/688338/EU
oaire.fundingStream5876
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccess
relation.isProjectOfPublicationf6c6102f-c577-4633-a70c-08a0b27e1207
relation.isProjectOfPublicationa5a1be12-8cdb-473c-9411-2cd9fc00b7f4
relation.isProjectOfPublication.latestForDiscoveryf6c6102f-c577-4633-a70c-08a0b27e1207

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