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Point-of-care testing of nitrite in oral medicine

2025-08Artigo científico Acesso aberto
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dc.contributor.authorGaspar, Sara Rodrigues
dc.contributor.authorProença, Luís
dc.contributor.authorAlves, Ricardo
dc.contributor.authorAlmeida, Maria Gabriela
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblElsevier BV
dc.date.accessioned2025-09-05T22:31:09Z
dc.date.available2025-09-05T22:31:09Z
dc.date.issued2025-08
dc.descriptionFunding Information: This work is financed by national funds through the FCT – Foundation for Science and Technology, I.P., under the projects UIDB/04585/2020 (Research Center - CiiEM), UIDP/04378/2020 and UIDB/04378/2020 (Research Unit on Applied Molecular Biosciences - UCIBIO), and LA/P/0140/2020 (Associate Laboratory Institute for Health and Bioeconomy - i4HB), and for the research project 2022.04940.PTDC. The authors acknowledge Nuno Costa from Laboratório de Análises/REQUIMTE/LAQV at NOVA FCT, Portugal, for the acquisition of the ion chromatography data. Publisher Copyright: © 2025 The Authors
dc.description.abstractPeriodontal disease (PD) is a chronic inflammatory condition triggered by bacterial biofilms and progresses through two main stages: gingivitis and periodontitis. The existing diagnostic methods remain time-consuming and require clinical expertise. Salivary nitrite, a stable end-product of nitric oxide produced during inflammation, has emerged as a promising biomarker for PD. However, its reliable quantification in saliva typically relies on laboratory-based assays that are unsuited to clinical workflows. This study aimed to test and validate a new nitrite point-of-care test (POCT) for oral medicine. Building on previous research, we developed a biosensing platform based on screen-printed carbon electrodes modified with a selective enzyme, the cytochrome c nitrite reductase. To adapt bioelectrodes for salivary analysis, they were further modified with a poly(vinyl) alcohol coating and a biochemical oxygen scavenger system (ascorbate oxidase and ascorbate). The nitrite biosensor achieved a suitable linear range of 5–300 μM, with a sensitivity of 0.015 μM−1, validated against the gold-standard Griess method. Unlike the Griess reaction, the biosensor was unaffected by sample turbidity, rendering centrifugation unnecessary. Additionally, we observed that sample freezing altered nitrite concentrations, increasing levels in non-centrifuged samples, while decreasing them in previously clarified samples. These findings highlight the need for real-time analysis and call into question the reliability of previously published data that overlooked these variables. Therefore, our results demonstrate the potential of the nitrite biosensor as a novel salivary POCT and emphasize the critical need for standardized sample-handling protocols.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent10
dc.format.extent4871062
dc.identifier.doi10.1016/j.sbsr.2025.100860
dc.identifier.issn2214-1804
dc.identifier.otherPURE: 128750520
dc.identifier.otherPURE UUID: 04b93e0e-3cc5-45ef-b35b-02832785287c
dc.identifier.otherScopus: 105012628995
dc.identifier.urihttp://hdl.handle.net/10362/187605
dc.identifier.urlhttps://www.scopus.com/pages/publications/105012628995
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04585%2F2020/PT
dc.relationEgas Moniz Interdisciplinary Research Center
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/2022.04940.PTDC/PT
dc.subjectBiosensors
dc.subjectNitrite
dc.subjectPeriodontal disease
dc.subjectPoint-of-care test
dc.subjectSaliva
dc.subjectBiotechnology
dc.subjectElectronic, Optical and Magnetic Materials
dc.subjectAnalytical Chemistry
dc.subjectSignal Processing
dc.subjectElectrical and Electronic Engineering
dc.subjectSDG 3 - Good Health and Well-being
dc.titlePoint-of-care testing of nitrite in oral medicineen
dc.title.subtitleApplication and validation of an enzymatic biosensor in human salivaen
dc.typejournal article
degois.publication.firstPage1
degois.publication.lastPage10
degois.publication.titleSensing and Bio-Sensing Research
degois.publication.volume49
dspace.entity.typePublication
oaire.awardNumberUIDB/04585/2020
oaire.awardNumberUIDP/04378/2020
oaire.awardNumberUIDB/04378/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardNumber2022.04940.PTDC
oaire.awardTitleEgas Moniz Interdisciplinary Research Center
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04585%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/2022.04940.PTDC/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication29698a2e-5c49-4923-aab9-3ffeee572562
relation.isProjectOfPublication38373452-5c9c-4724-8def-f03314ecce0e
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