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Prevalence and risk factor analysis of haemoplasmas infection in cats from Lahore (Pakistan)

2020-06Artigo científico Acesso aberto
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dc.contributor.authorSalim, Mubashra
dc.contributor.authorIbrahim, Omeira
dc.contributor.authorVilhena, Hugo
dc.contributor.authorMaia, Carla
dc.contributor.authorPereira, André
dc.contributor.authorShahzeen, Maria
dc.contributor.authorKalsoom, Shabana
dc.contributor.authorMahmood, Asim K.
dc.contributor.authorIqbal, Furhan
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.pblUniversity of Punjab (new Campus)
dc.date.accessioned2021-05-01T22:51:34Z
dc.date.available2021-05-01T22:51:34Z
dc.date.issued2020-06
dc.description.abstractHemotropic mycoplasmas (hemoplasmas) are obligate Gram-negative bacteria that target red blood cells, and infect a wide range of hosts including cats, dogs, domestic ruminants, pigs, rodents and humans. The present study was designed for the molecular detection of Mycoplasma haemofelis and Candidatus Mycoplasma haemominutum in feline blood samples collected from various pet clinics in Pakistan, by Polymerase Chain Reaction (PCR), using 16S rDNA as the target sequence. Clinical and epidemiological data was collected in all animals included in the study. M. haemofelis and C. Mycoplasma haemominutum DNA was detected by PCR respectively in 6.8% (10/148) and in 18.2% (27/148) of cat blood samples. Of these, two animals were co-infected with both agents. Sequencing and phylogenetic analysis was performed in M. haemofelis infected samples. Analysis of risk factors revealed that risk of M. haemofelis and of C. Mycoplasma haemominutum infection was significantly higher during summer months than during the winter season (P ≤ 0.01 in both agents). Cats older than one year of age were significantly more predisposed to C. Mycoplasma haemominutum infection than younger cats (P ≤ 0.001), and fever manifestation was significantly associated with M. haemofelis infection (P ≤ 0.001) in enrolled subjects. In conclusion, this study represents the first report of molecular detection of M. haemofelis and C. Mycoplasma haemominutum in cats from Pakistan. Therefore, this agents should be considered in cats from Pakistan presented with compatible clinical signs, mainly those presented during summer season, and in adult cats and cats presented with fever.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent4
dc.format.extent592633
dc.identifier.doi10.17582/JOURNAL.PJZ/20180210110232
dc.identifier.issn0030-9923
dc.identifier.otherPURE: 19899587
dc.identifier.otherPURE UUID: 195e16b3-22f6-4935-9598-a0d9d375ed65
dc.identifier.otherScopus: 85090465686
dc.identifier.otherORCID: /0000-0002-2545-7686/work/80678851
dc.identifier.otherWOS: 000521731600054
dc.identifier.urihttp://hdl.handle.net/10362/116614
dc.identifier.urlhttps://www.scopus.com/pages/publications/85090465686
dc.identifier.urlhttp://researcherslinks.com/current-issues/Prevalence-and-Risk-Factor-Analysis-of-Lahore/20/3/2802/html
dc.language.isoeng
dc.peerreviewedyes
dc.subject16S rDNA
dc.subjectCandidatus mycoplasma haemominutum
dc.subjectCat
dc.subjectMycoplasma haemofelis
dc.subjectPCR
dc.subjectAnimal Science and Zoology
dc.subjectEpidemiology
dc.subjectGeneral Veterinary
dc.subjectGenetics
dc.subjectSDG 3 - Good Health and Well-being
dc.titlePrevalence and risk factor analysis of haemoplasmas infection in cats from Lahore (Pakistan)en
dc.typejournal article
degois.publication.firstPage1213
degois.publication.issue3
degois.publication.lastPage1216
degois.publication.titlePakistan Journal of Zoology
degois.publication.volume52
dspace.entity.typePublication
rcaap.rightsopenAccess

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