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Respiratory viruses in mechanically ventilated patients

2020-02-13Artigo científico Acesso aberto
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dc.contributor.authorNazareth, Raquel
dc.contributor.authorChasqueira, Maria Jesus
dc.contributor.authorChasqueira, Maria-Jesus
dc.contributor.authorRodrigues, Maria Lúcia
dc.contributor.authorPaulino, Carolina
dc.contributor.authorConceição, Catarina
dc.contributor.authorLêdo, Lia
dc.contributor.authorSegura, Úrsula
dc.contributor.authorSantos, Madalena
dc.contributor.authorMessias, António
dc.contributor.authorPóvoa, Pedro
dc.contributor.authorPovoa, Pedro
dc.contributor.authorPaixão, Paulo
dc.contributor.authorPaixão, Paulo
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblBioMed Central (BMC)
dc.date.accessioned2020-02-21T23:54:34Z
dc.date.available2020-02-21T23:54:34Z
dc.date.issued2020-02-13
dc.description.abstractBACKGROUND: Respiratory virome is an integral part of the human microbiome and its characterization may contribute to a better understanding of the changes that arise in the disease and, consequently, influence the approach and treatment of patients with acute lower respiratory infections. The aim of this study was to evaluate the presence of respiratory viruses in the lower airways of individuals undergoing invasive mechanical ventilation, with and without acute lower respiratory infection (respectively WRI and WORI groups). METHODS: We studied 44 mini-bronchoalveolar lavage samples (collected with a double catheter, Combicath® kit) from patients with mean age in the seventh decade, 20 from WORI group and 24 from WRI group, who were hospitalized for acute respiratory failure in Intensive Care Units of two hospitals in the Lisbon area. Real-time PCR was applied to verify analyse the presence of 15 common respiratory viruses (adenovirus, human bocavirus, influenza virus A and B, repiratory syncytial virus, human parainfluenza virus types 1, 2, 3 and 4, human enterovirus, human rhinovirus, human metapneumovirus, human coronavirus group 1 (229E, NL63) and 2 (OC43, HKU1). RESULTS: Respiratory viruses were detected in six of the 20 patients in the WORI group: influenza AH3 (n = 2), parainfluenza virus 1/3 (n = 2), human rhinovirus (n = 2), respiratory syncytial virus (n = 1) and human metapneumovirus (n = 1). In the WRI group, respiratory viruses were detected in 12 of the 24 patients: influenza AH3 (n = 3), human rhinovirus (n = 3), respiratory syncytial virus (n = 3), human metapneumovirus (n = 3), human bocavirus (n = 2) and human enterovirus (n = 1). Simultaneous detection of two viruses was recorded in two samples in both groups. CONCLUSIONS: The results of this study suggest the presence of common respiratory viruses in the lower respiratory tract without causing symptomatic infection, even in carefully collected lower samples. This may have important implications on the interpretation of the results on the diagnostic setting.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1
dc.format.extent497004
dc.identifier.doi10.1186/s12890-020-1082-5
dc.identifier.issn1471-2466
dc.identifier.otherPURE: 16971086
dc.identifier.otherPURE UUID: 9b6ffc49-737f-4d16-ba1d-04954f6270b1
dc.identifier.otherScopus: 85079339566
dc.identifier.otherPubMed: 32054471
dc.identifier.otherWOS: 000514921200001
dc.identifier.urihttp://hdl.handle.net/10362/93191
dc.identifier.urlhttps://www.scopus.com/pages/publications/85079339566
dc.language.isoeng
dc.peerreviewedyes
dc.subjectHuman microbiome
dc.subjectReal-time PCR
dc.subjectRespiratory virome
dc.subjectRespiratory viruses
dc.subjectPulmonary and Respiratory Medicine
dc.subjectSDG 3 - Good Health and Well-being
dc.titleRespiratory viruses in mechanically ventilated patientsen
dc.title.subtitlea pilot studyen
dc.typejournal article
degois.publication.issue1
degois.publication.titleBMC Pulmonary Medicine
degois.publication.volume20
dspace.entity.typePublication
person.familyNameChasqueira
person.familyNamePovoa
person.familyNamePaixão
person.givenNameMaria-Jesus
person.givenNamePedro
person.givenNamePaulo
person.identifier.ciencia-id0C16-5CF9-9238
person.identifier.orcid0000-0002-8015-7109
person.identifier.orcid0000-0002-7069-7304
person.identifier.orcid0000-0002-4294-0872
person.identifier.scopus-author-id6602772147
person.identifier.scopus-author-id6508103792
rcaap.rightsopenAccess
relation.isAuthorOfPublication072ec2a7-7dea-429d-bdb3-2d9fa3f1e79d
relation.isAuthorOfPublication04ec38ba-be1e-46e5-8007-0e65a557d0f4
relation.isAuthorOfPublication05b765bd-f025-43ee-8cf5-2e54ceb007cb
relation.isAuthorOfPublication.latestForDiscovery072ec2a7-7dea-429d-bdb3-2d9fa3f1e79d

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