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Histological characterization of downy mildew infection in wild rocket (Diplotaxis tenuifolia)

2024-08-02Artigo científico Acesso aberto
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dc.contributor.authorPereira, Ana L.
dc.contributor.authorScotti-Campos, Paula
dc.contributor.authorCoelho, Paula S.
dc.contributor.institutionGeoBioTec - Geobiociências, Geoengenharias e Geotecnologias
dc.contributor.institutionFaculdade de Ciências e Tecnologia (FCT)
dc.contributor.institutionBioresources 4 Sustainability (GREEN-IT)
dc.contributor.pblBlackwell Publishing Ltd
dc.date.accessioned2024-10-07T22:24:29Z
dc.date.available2024-10-07T22:24:29Z
dc.date.issued2024-08-02
dc.descriptionFunding Information: This work was supported by the project PTDC/ASP-PLA/28963/2017 and ALG-01-0145-FEDER-028963 (REMIRUCULA\u2014Resistance characterization to downy mildew in wild rocket crop) and the FCT\u2014Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia, IP, through the R&D Unit \u2018GREEN-IT\u2013Bioresources for Sustainability\u2019 UIDB/04551/2020 (https://doi.org/10.54499/UIDB/04551/2020) and UIDP/04551/2020 (https://doi.org/10.54499/UIDP/04551/2020).
dc.description.abstractWild rocket downy mildew (DM), caused by oomycete Hyaloperonospora sp., is a widely spread disease reducing crop production and quality. New productive wild rocket varieties resistant to DM are crucial to control disease and ensure high quality leaves. A histological characterization of Hyaloperonospora sp. infection was performed in 11 wild rocket (Diplotaxis tenuifolia) accessions with contrasting DM responses (R, resistant; PR, partially resistant; S, susceptible). Samples of infected cotyledons, first and second leaves of 14-day-old seedlings were collected at 3, 6, 9, 21 and 24 h post-inoculation (hpi) and 7 days post-inoculation (dpi) and stained for appressoria and haustoria observation and tissue necrosis evaluation. Occurrence of appressoria on host surfaces was higher in leaves compared with cotyledons but unrelated with DM resistance response. Haustoria growth in mycelium was delayed in R accessions from 3 hpi, and signs of cell hypersensitivity reaction were observed at 9 hpi. At 24 hpi, necrotic spots limited pathogen growth in resistant accessions, whilst mycelium invaded larger mesophyll areas and produced more haustoria in susceptible ones. At 7 dpi, sporulation was heavy in S, restricted to droplet deposition sites in PR, and did not occur in R hosts. A rapid response of R accessions resulted in slower mycelium growth and longer infection periods. These traits have important agronomic value and should be considered in germplasm selection for breeding programmes aimed at crop protection. A better understanding of the host response to DM infection will allow selection of more suitable wild rocket accessions in future breeding programmes.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent12
dc.format.extent2515314
dc.identifier.doi10.1111/ppa.13979
dc.identifier.issn0032-0862
dc.identifier.otherPURE: 100449215
dc.identifier.otherPURE UUID: c44e2aa7-47bc-47be-b640-214438e65333
dc.identifier.otherScopus: 85200260572
dc.identifier.urihttp://hdl.handle.net/10362/173119
dc.identifier.urlhttps://www.scopus.com/pages/publications/85200260572
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBrassicaceae
dc.subjectdowny mildew resistance
dc.subjecthistology
dc.subjectHyaloperonospora sp.
dc.subjectplant–pathogen interaction
dc.subjectseedling stage
dc.subjectGenetics
dc.subjectAgronomy and Crop Science
dc.subjectPlant Science
dc.subjectHorticulture
dc.titleHistological characterization of downy mildew infection in wild rocket (Diplotaxis tenuifolia)en
dc.typejournal article
degois.publication.firstPage2516
degois.publication.issue9
degois.publication.lastPage2527
degois.publication.titlePlant Pathology
degois.publication.volume73
dspace.entity.typePublication
rcaap.rightsopenAccess

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